Application
Western blot analysis of TRAF2 (arrow) using rabbit polyclonal TRAF2 Antibody (Center) . 293 cell lysates (2 ug/lane) either nontransfected (c) or transiently transfected with the TRAF2 gene (Lane 2) (Origene Technologies).Application
Formalin-fixed and paraffin-embedded human colon carcinoma tissue reacted with TRAF2 antibody (Center) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.| Product Name | TRAF2 Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | TRAF2; TRAP3; TNF receptor-associated factor 2; E3 ubiquitin-protein ligase TRAF2; Tumor necrosis factor type 2 receptor-associated protein 3 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:50~100 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | TRAP3 |
|---|---|
| Alternative Names | TRAF2 TRAP3 TNF receptor-associated factor 2 E3 ubiquitin-protein ligase TRAF2 Tumor necrosis factor type 2 receptor-associated protein 3 |
| Molecular Weight(MW) | 55859 Da |
| Function | Regulates activation of NF-kappa-B and JNK and plays a central role in the regulation of cell survival and apoptosis Required for normal antibody isotype switching from IgM to IgG Has E3 ubiquitin-protein ligase activity and promotes 'Lys-63'- linked ubiquitination of target proteins, such as BIRC3, RIPK1 and TICAM1. Is an essential constituent of several E3 ubiquitin- protein ligase complexes, where it promotes the ubiquitination of target proteins by bringing them into contact with other E3 ubiquitin ligases. Regulates BIRC2 and BIRC3 protein levels by inhibiting their autoubiquitination and subsequent degradation; this does not depend on the TRAF2 RING-type zinc finger domain |
| Tissue Specificity | This TRAF2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 132~162 amino acids from the center region of human TRAF2. |
| Cellular Localization | Cytoplasm. |
| Entrez Gene | 7186 |
|---|
Application
Western blot analysis of TRAF2 (arrow) using rabbit polyclonal TRAF2 Antibody (Center) . 293 cell lysates (2 ug/lane) either nontransfected (c) or transiently transfected with the TRAF2 gene (Lane 2) (Origene Technologies).
Application
Formalin-fixed and paraffin-embedded human colon carcinoma tissue reacted with TRAF2 antibody (Center) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.| Application Notes | WB:1:50~100 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. |
