Application
Confocal immunofluorescent analysis of TRAF2 Antibody (Cat#AM1895b) with MCF-7 cell followed by Alexa Fluor 488-conjugated goat anti-mouse lgG (green). DAPI was used to stain the cell nuclear (blue).Application
TRAF2/MB10188 antibody (Cat. #AM1895b) western blot analysis in T47D cell line lysates (35ug/lane).This demonstrates the TRAF2/MB10188 antibody detected the TRAF2/MB10188 protein (arrow).| Product Name | TRAF2 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | TRAF2; TRAP3; TNF receptor-associated factor 2; E3 ubiquitin-protein ligase TRAF2; Tumor necrosis factor type 2 receptor-associated protein 3 |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 214CT16.3.4 |
| Host Species | Mouse |
| Tested Applications | IFWB |
| IF:1:10~50 WB |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | TRAP3 |
|---|---|
| Alternative Names | TRAF2 TRAP3 TNF receptor-associated factor 2 E3 ubiquitin-protein ligase TRAF2 Tumor necrosis factor type 2 receptor-associated protein 3 |
| Molecular Weight(MW) | 55859 Da |
| Function | Regulates activation of NF-kappa-B and JNK and plays a central role in the regulation of cell survival and apoptosis Required for normal antibody isotype switching from IgM to IgG Has E3 ubiquitin-protein ligase activity and promotes 'Lys-63'- linked ubiquitination of target proteins, such as BIRC3, RIPK1 and TICAM1. Is an essential constituent of several E3 ubiquitin- protein ligase complexes, where it promotes the ubiquitination of target proteins by bringing them into contact with other E3 ubiquitin ligases. Regulates BIRC2 and BIRC3 protein levels by inhibiting their autoubiquitination and subsequent degradation; this does not depend on the TRAF2 RING-type zinc finger domain |
| Tissue Specificity | This TRAF2 monoclonal antibody is generated from mouse immunized with TRAF2 recombinant protein. |
| Cellular Localization | Cytoplasm. |
| Entrez Gene | 7186 |
|---|---|
| Nucleotide Accession | NP_066961.2 |
Application
Confocal immunofluorescent analysis of TRAF2 Antibody (Cat#AM1895b) with MCF-7 cell followed by Alexa Fluor 488-conjugated goat anti-mouse lgG (green). DAPI was used to stain the cell nuclear (blue).
Application
TRAF2/MB10188 antibody (Cat. #AM1895b) western blot analysis in T47D cell line lysates (35ug/lane).This demonstrates the TRAF2/MB10188 antibody detected the TRAF2/MB10188 protein (arrow).| Application Notes | IF:1:10~50 WB |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. |
