Application
Immunofluorescence of monoclonal antibody to TRIM28 (AT4348a) on HeLa cell . [antibody concentration 10 ug/ml]Application
Western blot analysis of TRIM28 over-expressed 293 cell line, cotransfected with TRIM28 Validated Chimera RNAi ( Cat # H00010155-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with TRIM28 monoclonal antibody (M01) clone 4E6 (Cat # AT4348a ). GAPDH ( 36.1 kDa ) used as specificity and loading control.| Product Name | TRIM28 Antibody (monoclonal) (M01) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | KAP1, RNF96, TF1B, TIF1Btripartite motif-containing 28 |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2b |
| Clone Number | 4000000 |
| Host Species | Mouse |
| Tested Applications | IFWB |
| WB:1:500~1000 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | KAP1 RNF96 TF1B TIF1Btripartite motif-containing 28 |
|---|---|
| Molecular Weight(MW) | 88550 Da |
| Tissue Specificity | TRIM28 (AAH04978, 379 a.a. ~ 524 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. |
| Entrez Gene | 10155 |
|---|
Application
Immunofluorescence of monoclonal antibody to TRIM28 (AT4348a) on HeLa cell . [antibody concentration 10 ug/ml]
Application
Western blot analysis of TRIM28 over-expressed 293 cell line, cotransfected with TRIM28 Validated Chimera RNAi ( Cat # H00010155-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with TRIM28 monoclonal antibody (M01) clone 4E6 (Cat # AT4348a ). GAPDH ( 36.1 kDa ) used as specificity and loading control.| Application Notes | WB:1:500~1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Clear, colorless solution in phosphate buffered saline, pH 7.2 . |
