Application
Western blot analysis on (A) JAR and (B) 293T cell lysates using anti-Tryptophanyl-tRNA synthetase RabMAb (Cat. #AJ1796a).Application
Immunofluorescent staining of HeLa cells using anti-Tryptophanyl-tRNA synthetase RabMAb (Cat. #AJ1796a).| Product Name | Tryptophanyl-tRNA Synthase Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | WARS, IFI53, WRS, Tryptophanyl-tRNA synthetase, cytoplasmic, Interferon-induced protein 53 |
| Clonality | Monoclonal |
|---|---|
| Clone Number | EPR3424 |
| Host Species | Rabbit |
| Tested Applications | WBIF |
| WB:1:50000~200000 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | IFI53 WRS |
|---|---|
| Alternative Names | WARS IFI53 WRS Tryptophanyl-tRNA synthetase cytoplasmic Interferon-induced protein 53 |
| Molecular Weight(MW) | 53165 Da |
| Function | Isoform 1, isoform 2 and T1-TrpRS have aminoacylation activity while T2-TrpRS lacks it. Isoform 2, T1-TrpRS and T2-TrpRS possess angiostatic activity whereas isoform 1 lacks it. T2-TrpRS inhibits fluid shear stress-activated responses of endothelial cells. Regulates ERK, Akt, and eNOS activation pathways that are associated with angiogenesis, cytoskeletal reorganization and shear stress-responsive gene expression |
| Tissue Specificity | A synthetic peptide corresponding to residues at the C terminus of human Tryptophanyl-tRNA synthetase was used as an immunogen. |
| Cellular Localization | Cytoplasm. |
| Entrez Gene | 7453 |
|---|
Application
Western blot analysis on (A) JAR and (B) 293T cell lysates using anti-Tryptophanyl-tRNA synthetase RabMAb (Cat. #AJ1796a).
Application
Immunofluorescent staining of HeLa cells using anti-Tryptophanyl-tRNA synthetase RabMAb (Cat. #AJ1796a).| Application Notes | WB:1:50000~200000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. |
