Application
Western blot analysis of WARS Antibody (N-term) in NCI-H460 cell line lysates (35ug/lane). WARS (arrow) was detected using the purified Pab.Application
Formalin-fixed and paraffin-embedded human colon carcinoma reacted with WARS Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.Application
Flow cytometric analysis of K562 cells using WARS Antibody (N-term)(bottom histogram) compared to a negative control cell (top histogram)FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | WARS Antibody (N-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | WARS; IFI53; WRS; Tryptophan--tRNA ligase, cytoplasmic; Interferon-induced protein 53; Tryptophanyl-tRNA synthetase; T1-TrpRS; T2-TrpRS |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCFC |
| WB:1:50~100 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | IFI53 WRS |
|---|---|
| Alternative Names | WARS IFI53 WRS Tryptophan--tRNA ligase cytoplasmic Interferon-induced protein 53 Tryptophanyl-tRNA synthetase T1-TrpRS T2-TrpRS |
| Molecular Weight(MW) | 53165 Da |
| Function | Isoform 1, isoform 2 and T1-TrpRS have aminoacylation activity while T2-TrpRS lacks it. Isoform 2, T1-TrpRS and T2-TrpRS possess angiostatic activity whereas isoform 1 lacks it. T2-TrpRS inhibits fluid shear stress-activated responses of endothelial cells. Regulates ERK, Akt, and eNOS activation pathways that are associated with angiogenesis, cytoskeletal reorganization and shear stress-responsive gene expression |
| Tissue Specificity | This WARS antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 103~132 amino acids from the N-terminal region of human WARS. |
| Cellular Localization | Cytoplasm. |
| Entrez Gene | 7453 |
|---|
Application
Western blot analysis of WARS Antibody (N-term) in NCI-H460 cell line lysates (35ug/lane). WARS (arrow) was detected using the purified Pab.
Application
Formalin-fixed and paraffin-embedded human colon carcinoma reacted with WARS Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Application
Flow cytometric analysis of K562 cells using WARS Antibody (N-term)(bottom histogram) compared to a negative control cell (top histogram)FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:50~100 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
