Application
Western blot analysis of TWIST1 over-expressed 293 cell line, cotransfected with TWIST1 Validated Chimera RNAi ( Cat # H00007291-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with TWIST1 monoclonal antibody (M01), clone 3E11 (Cat # AT4410a ). GAPDH ( 36.1 kDa ) used as specificity and loading control.Application
Western Blot analysis of TWIST1 expression in transfected 293T cell line by TWIST1 monoclonal antibody (M01), clone 3E11.Lane 1: TWIST1 transfected lysate(21 KDa).Lane 2: Non-transfected lysate.| Product Name | TWIST1 Antibody (monoclonal) (M01) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | ACS3, BPES2, BPES3, SCS, TWISTtwist homolog 1 (acrocephalosyndactyly 3; Saethre-Chotzen syndrome) (Drosophila) |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 300000000000 |
| Host Species | Mouse |
| Tested Applications | WB |
| WB:1:500~1000 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | ACS3 BPES2 BPES3 SCS TWISTtwist homolog 1 (acrocephalosyndactyly 3 Saethre-Chotzen syndrome) (Drosophila) |
|---|---|
| Molecular Weight(MW) | 20954 Da |
| Tissue Specificity | TWIST1 (NP_000465, 100 a.a. ~ 203 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. |
Application
Western blot analysis of TWIST1 over-expressed 293 cell line, cotransfected with TWIST1 Validated Chimera RNAi ( Cat # H00007291-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with TWIST1 monoclonal antibody (M01), clone 3E11 (Cat # AT4410a ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
Application
Western Blot analysis of TWIST1 expression in transfected 293T cell line by TWIST1 monoclonal antibody (M01), clone 3E11.Lane 1: TWIST1 transfected lysate(21 KDa).Lane 2: Non-transfected lysate.| Application Notes | WB:1:500~1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Clear, colorless solution in phosphate buffered saline, pH 7.2 . |
