Application
Figure 1: Western blot analysis using anti-TYRO3 monoclonal antiobdy against truncated TYRO3 recombinant protein.Application
Figure 2: Immunofluorescence staining of methanol-fixed MCF-7 and HepG2 cells showing membrane and cytoplasmic localization.| Product Name | TYRO3 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | BYK; Brt; Dtk; RSE; Sky; Tif; FLJ16467 |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 1C10E8, 9A3F2 |
| Host Species | Mouse |
| Tested Applications | WBIF |
| WB:1:500 - 2000. IF |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | BYK DTK RSE SKY TIF |
|---|---|
| Alternative Names | BYK Brt Dtk RSE Sky Tif FLJ16467 |
| Molecular Weight(MW) | 96905 Da |
| Function | Receptor tyrosine kinase that transduces signals from the extracellular matrix into the cytoplasm by binding to several ligands including TULP1 or GAS6. Regulates many physiological processes including cell survival, migration and differentiation Ligand binding at the cell surface induces dimerization and autophosphorylation of TYRO3 on its intracellular domain that provides docking sites for downstream signaling molecules Following activation by ligand, interacts with PIK3R1 and thereby enhances PI3-kinase activity. Activates the AKT survival pathway, including nuclear translocation of NF-kappa-B and up-regulation of transcription of NF-kappa-B-regulated genes. TYRO3 signaling plays a role in various processes such as neuron protection from excitotoxic injury, platelet aggregation and cytoskeleton reorganization. Plays also an important role in inhibition of Toll-like receptors (TLRs)-mediated innate immune response by activating STAT1, which selectively induces production of suppressors of cytokine signaling SOCS1 and SOCS3 |
| Tissue Specificity | Abundant in the brain and lower levels in other tissues |
| Cellular Localization | Cell membrane; Single-pass type I membrane protein |
| Entrez Gene | 7301 |
|---|---|
| Nucleotide Accession | NP_006284.2 |
Application
Figure 1: Western blot analysis using anti-TYRO3 monoclonal antiobdy against truncated TYRO3 recombinant protein.
Application
Figure 2: Immunofluorescence staining of methanol-fixed MCF-7 and HepG2 cells showing membrane and cytoplasmic localization.| Application Notes | WB:1:500 - 2000. IF |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified recombinant fragment of TYRO3 expressed in E. Coli. |
