Application
UCHL1 Antibody(Cat. #AM1959b) western blot analysis in NCI-H460 cell line lysates (35ug/lane).This demonstrates the UCHL1 antibody detected the UCHL1 protein (arrow).| Product Name | UCHL1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | UCHL1; Ubiquitin carboxyl-terminal hydrolase isozyme L1; Neuron cytoplasmic protein 9.5; PGP 9.5; Ubiquitin thioesterase L1 |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 346CT2.2.1 |
| Host Species | Mouse |
| Tested Applications | WB |
| WB:1:120~1000 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | UCHL1 Ubiquitin carboxyl-terminal hydrolase isozyme L1 Neuron cytoplasmic protein 9.5 PGP 9.5 Ubiquitin thioesterase L1 |
|---|---|
| Molecular Weight(MW) | 24824 Da |
| Function | Ubiquitin-protein hydrolase involved both in the processing of ubiquitin precursors and of ubiquitinated proteins This enzyme is a thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin. Also binds to free monoubiquitin and may prevent its degradation in lysosomes The homodimer may have ATP-independent ubiquitin ligase activity |
| Tissue Specificity | Found in neuronal cell bodies and processes throughout the neocortex (at protein level). Expressed in neurons and cells of the diffuse neuroendocrine system and their tumors Weakly expressed in ovary. Down-regulated in brains from Parkinson disease and Alzheimer disease patients |
| Cellular Localization | Cytoplasm. Endoplasmic reticulum membrane; Lipid-anchor. Note=About 30% of total UCHL1 is associated with membranes in brain |
| Entrez Gene | 7345 |
|---|---|
| Nucleotide Accession | NP_004172.2 |
Application
UCHL1 Antibody(Cat. #AM1959b) western blot analysis in NCI-H460 cell line lysates (35ug/lane).This demonstrates the UCHL1 antibody detected the UCHL1 protein (arrow).| Application Notes | WB:1:120~1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. |
