Application
Figure 1: Western blot analysis using anti-Vimentin monoclonal antibody against Hela (1), COS(2), HEK293 (3) and U20S (4) cell lysate.Application
Figure 2: Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue, showing cytoplasmic localization using anti-Vimentin antibody with DAB staining.| Product Name | Vimentin Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 4F2E9 |
| Host Species | Mouse |
| Tested Applications | WBIHC |
| WB:1:500 - 2000. IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | VIM |
|---|---|
| Molecular Weight(MW) | 53652 Da |
| Function | Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally |
| Tissue Specificity | Highly expressed in fibroblasts, some expression in T- and B-lymphocytes, and little or no expression in Burkitt's lymphoma cell lines. Expressed in many hormone- independent mammary carcinoma cell lines |
| Cellular Localization | Cytoplasm. |
| Entrez Gene | 7431 |
|---|---|
| Nucleotide Accession | NP_003371.2 |
Application
Figure 1: Western blot analysis using anti-Vimentin monoclonal antibody against Hela (1), COS(2), HEK293 (3) and U20S (4) cell lysate.
Application
Figure 2: Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue, showing cytoplasmic localization using anti-Vimentin antibody with DAB staining.| Application Notes | WB:1:500 - 2000. IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified recombinant fragment of Vimentin(aa2-466) expressed in E. Coli. |
