Application
A. Western blot analysis on Jurkat cell lysate using anti-VLA-alpha4 RabMAb (Cat. #AJ1818a), dilution 1:2000.Application
B. Immunohistochemical staining of paraffin-embedded human tonsils using anti-VLA-alpha4 RabMAb (Cat. #AJ1818a).| Product Name | VLA alpha 4 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | ITGA4, CD49D, Integrin alpha-4, Integrin alpha-IV;VLA-4;CD49 antigen-like family member D |
| Clonality | Monoclonal |
|---|---|
| Clone Number | EPR1355Y |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:1000~2000 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | CD49D |
|---|---|
| Alternative Names | ITGA4 CD49D Integrin alpha-4 Integrin alpha-IV VLA-4 CD49 antigen-like family member D |
| Molecular Weight(MW) | 114900 Da |
| Function | Integrins alpha-4/beta-1 (VLA-4) and alpha-4/beta-7 are receptors for fibronectin. They recognize one or more domains within the alternatively spliced CS-1 and CS-5 regions of fibronectin. They are also receptors for VCAM1. Integrin alpha- 4/beta-1 recognizes the sequence Q-I-D-S in VCAM1. Integrin alpha- 4/beta-7 is also a receptor for MADCAM1. It recognizes the sequence L-D-T in MADCAM1. On activated endothelial cells integrin VLA-4 triggers homotypic aggregation for most VLA-4-positive leukocyte cell lines. It may also participate in cytolytic T-cell interactions with target cells |
| Tissue Specificity | A synthetic peptide corresponding to residues near the C-terminus of human VLA-alpha 4 was used as an immunogen. |
| Cellular Localization | Membrane; Single-pass type I membrane protein |
| Entrez Gene | 3676 |
|---|
Application
A. Western blot analysis on Jurkat cell lysate using anti-VLA-alpha4 RabMAb (Cat. #AJ1818a), dilution 1:2000.
Application
B. Immunohistochemical staining of paraffin-embedded human tonsils using anti-VLA-alpha4 RabMAb (Cat. #AJ1818a).| Application Notes | WB:1:1000~2000 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. |
