Application
A. Western blot analysis on THP-1 cell lysates using anti-Phospho-Integrin alpha 4 (pS1027) RabMAb (Cat. #AJ1409a), 1:2000 dilution. Cells were either (A) untreated (B) treated with Lambda Phosphatase| Product Name | Integrin-alpha4 Antibody Phospho-ps1027 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | ITGA4, CD49D, Integrin alpha-4, Integrin alpha-IV;VLA-4;CD49 antigen-like family member D |
| Modification | p-s1027 |
| Clonality | Monoclonal |
|---|---|
| Clone Number | EPR2343 |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:500~2000 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | CD49D |
|---|---|
| Alternative Names | ITGA4 CD49D Integrin alpha-4 Integrin alpha-IV VLA-4 CD49 antigen-like family member D |
| Molecular Weight(MW) | 114900 Da |
| Function | Integrins alpha-4/beta-1 (VLA-4) and alpha-4/beta-7 are receptors for fibronectin. They recognize one or more domains within the alternatively spliced CS-1 and CS-5 regions of fibronectin. They are also receptors for VCAM1. Integrin alpha- 4/beta-1 recognizes the sequence Q-I-D-S in VCAM1. Integrin alpha- 4/beta-7 is also a receptor for MADCAM1. It recognizes the sequence L-D-T in MADCAM1. On activated endothelial cells integrin VLA-4 triggers homotypic aggregation for most VLA-4-positive leukocyte cell lines. It may also participate in cytolytic T-cell interactions with target cells |
| Tissue Specificity | A phospho-specific peptide corresponding to residues on human Integrin alpha 4 was used as an immunogen. The antibody only detects Integrin alpha4 phosphorylated on Serine 1027. The antibody detects the mature chain (180 kDa), the precursor (140 kDa) and the cleaved C terminal (70 kDa). |
| Cellular Localization | Membrane; Single-pass type I membrane protein |
| Entrez Gene | 3676 |
|---|
Application
A. Western blot analysis on THP-1 cell lysates using anti-Phospho-Integrin alpha 4 (pS1027) RabMAb (Cat. #AJ1409a), 1:2000 dilution. Cells were either (A) untreated (B) treated with Lambda Phosphatase| Application Notes | WB:1:500~2000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. |
