Application
Western blot analysis of WAS Antibody (Center) in mouse liver tissue lysates (35ug/lane). WAS (arrow) was detected using the purified Pab.Application
WAS Antibody (Center) IHC analysis in formalin fixed and paraffin embedded tonsil tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the WAS Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.Application
WAS Antibody (Center) flow cytometric analysis of CEM cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | WAS Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | WAS; IMD2; Wiskott-Aldrich syndrome protein |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCFC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | IMD2 |
|---|---|
| Alternative Names | WAS IMD2 Wiskott-Aldrich syndrome protein |
| Molecular Weight(MW) | 52913 Da |
| Function | Effector protein for Rho-type GTPases. Regulates actin filament reorganization via its interaction with the Arp2/3 complex. Important for efficient actin polymerization. Possible regulator of lymphocyte and platelet function. Mediates actin filament reorganization and the formation of actin pedestals upon infection by pathogenic bacteria |
| Tissue Specificity | Expressed predominantly in the thymus. Also found, to a much lesser extent, in the spleen |
| Cellular Localization | Cytoplasm, cytoskeleton. |
| Entrez Gene | 7454 |
|---|
Application
Western blot analysis of WAS Antibody (Center) in mouse liver tissue lysates (35ug/lane). WAS (arrow) was detected using the purified Pab.
Application
WAS Antibody (Center) IHC analysis in formalin fixed and paraffin embedded tonsil tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the WAS Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
Application
WAS Antibody (Center) flow cytometric analysis of CEM cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
