Application
Figure 1: Western blot analysis using anti-WNT5A monoclonal antibody against HEK293 (1) and WNT5A-hIgGFc transfected HEK293 cell lysate(2).Application
Figure 2: Immunohistochemical analysis of paraffin-embedded human lung cancer(A), thyroid cancer(B), lymph node(C) and brain(D) showing cytoplasmic and extracellular matrix localization using anti-WNT5A antibody with DAB staining.Application
Figure 3: Confocal immunofluorescence analysis of PC-12 cells using anti-WNT5A monoclonal antioby(green), showing cytoplasmic localization. Blue: DRAQ5 fluorescent DNA dye.| Product Name | WNT5A Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | hWNT5A |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 6F2 |
| Host Species | Mouse |
| Tested Applications | WBIHCIF |
| WB:1:500 - 2000. IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | hWNT5A |
|---|---|
| Molecular Weight(MW) | 42339 Da |
| Function | Ligand for members of the frizzled family of seven transmembrane receptors. Can activate or inhibit canonical Wnt signaling, depending on receptor context. In the presence of FZD4, activates beta-catenin signaling. In the presence of ROR2, inhibits the canonical Wnt pathway by promoting beta-catenin degradation through a GSK3-independent pathway which involves down-regulation of beta-catenin-induced reporter gene expression Suppression of the canonical pathway allows chondrogenesis to occur and inhibits tumor formation. Stimulates cell migration Decreases proliferation, migration, invasiveness and clonogenicity of carcinoma cells and may act as a tumor suppressor. Mediates motility of melanoma cells. Required during embryogenesis for extension of the primary anterior-posterior axis and for outgrowth of limbs and the genital tubercle. Inhibits type II collagen expression in chondrocytes |
| Tissue Specificity | Expression is increased in differentiated thyroid carcinomas compared to normal thyroid tissue and anaplastic thyroid tumors where expression is low or undetectable Expression is found in thyrocytes but not in stromal cells (at protein level) |
| Cellular Localization | Secreted, extracellular space, extracellular matrix |
| Entrez Gene | 7474 |
|---|---|
| Nucleotide Accession | NP_003383.2 |
Application
Figure 1: Western blot analysis using anti-WNT5A monoclonal antibody against HEK293 (1) and WNT5A-hIgGFc transfected HEK293 cell lysate(2).
Application
Figure 2: Immunohistochemical analysis of paraffin-embedded human lung cancer(A), thyroid cancer(B), lymph node(C) and brain(D) showing cytoplasmic and extracellular matrix localization using anti-WNT5A antibody with DAB staining.
Application
Figure 3: Confocal immunofluorescence analysis of PC-12 cells using anti-WNT5A monoclonal antioby(green), showing cytoplasmic localization. Blue: DRAQ5 fluorescent DNA dye.| Application Notes | WB:1:500 - 2000. IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Ascitic fluid containing 0.03% sodium azide. |
