Application
Western blot analysis of WNT10B Antibody (Center) in MDA-MB231 cell line lysates (35ug/lane). WNT10B (arrow) was detected using the purified Pab.Application
Western blot analysis of WNT10B Antibody (Center) in mouse liver tissue lysates (35ug/lane). WNT10B (arrow) was detected using the purified Pab.Application
WNT10B Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human colon carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the WNT10B Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.Application
Flow cytometric analysis of MDA-231 cells using WNT10B Antibody (Center)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | WNT10B Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | WNT10B; WNT12; Protein Wnt-10b; Protein Wnt-12 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCFC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | WNT12 |
|---|---|
| Alternative Names | WNT10B WNT12 Protein Wnt-10b Protein Wnt-12 |
| Molecular Weight(MW) | 43000 Da |
| Function | Ligand for members of the frizzled family of seven transmembrane receptors. Probable developmental protein. May be a signaling molecule which affects the development of discrete regions of tissues. Is likely to signal over only few cell diameters (By similarity) |
| Tissue Specificity | Detected in most adult tissues. Highest levels were found in heart and skeletal muscle. Low levels are found in brain |
| Cellular Localization | Secreted, extracellular space, extracellular matrix |
| Entrez Gene | 7480 |
|---|
Application
Western blot analysis of WNT10B Antibody (Center) in MDA-MB231 cell line lysates (35ug/lane). WNT10B (arrow) was detected using the purified Pab.
Application
Western blot analysis of WNT10B Antibody (Center) in mouse liver tissue lysates (35ug/lane). WNT10B (arrow) was detected using the purified Pab.
Application
WNT10B Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human colon carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the WNT10B Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
Application
Flow cytometric analysis of MDA-231 cells using WNT10B Antibody (Center)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
