Application
WWOX Antibody (Y287) western blot analysis in Jurkat cell line lysates (35ug/lane).This demonstrates the WWOX antibody detected the WWOX protein (arrow).Application
Confocal immunofluorescent analysis of WWOX Antibody (Center Y287)(Cat#AP14312c) with MCF-7 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).Application
WWOX Antibody (Center Y287) (AP14312c)immunohistochemistry analysis in formalin fixed and paraffin embedded human testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of WWOX Antibody (Center Y287) for immunohistochemistry. Clinical relevance has not been evaluated.| Product Name | WWOX Antibody (Center Y287) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | WWOX; FOR; WOX1; WW domain-containing oxidoreductase; Fragile site FRA16D oxidoreductase |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIFIHC |
| WB:1:100~500 IF |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | FOR WOX1 |
|---|---|
| Alternative Names | WWOX FOR WOX1 WW domain-containing oxidoreductase Fragile site FRA16D oxidoreductase |
| Molecular Weight(MW) | 46677 Da |
| Function | Putative oxidoreductase. Acts as a tumor suppressor and plays a role in apoptosis. Required for normal bone development (By similarity). May function synergistically with p53/TP53 to control genotoxic stress-induced cell death. Plays a role in TGFB1 signaling and TGFB1-mediated cell death. May also play a role in tumor necrosis factor (TNF)-mediated cell death. Inhibits Wnt signaling, probably by sequestering DVL2 in the cytoplasm |
| Tissue Specificity | Widely expressed. Strongly expressed in testis, prostate, and ovary. Overexpressed in cancer cell lines Isoform 5 and isoform 6 may only be expressed in tumor cell lines |
| Cellular Localization | Cytoplasm. Nucleus. Mitochondrion. Golgi apparatus. Note=Partially localizes to the mitochondria Translocates to the nucleus upon genotoxic stress or TNF stimulation (By similarity). Translocates to the nucleus in response to TGFB1. Isoform 5 and isoform 6 may localize in the nucleus |
| Entrez Gene | 51741 |
|---|---|
| Nucleotide Accession | NP_057457.1 NP_570859.1 NP_570607.1 |
Application
WWOX Antibody (Y287) western blot analysis in Jurkat cell line lysates (35ug/lane).This demonstrates the WWOX antibody detected the WWOX protein (arrow).
Application
Confocal immunofluorescent analysis of WWOX Antibody (Center Y287)(Cat#AP14312c) with MCF-7 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).
Application
WWOX Antibody (Center Y287) (AP14312c)immunohistochemistry analysis in formalin fixed and paraffin embedded human testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of WWOX Antibody (Center Y287) for immunohistochemistry. Clinical relevance has not been evaluated.| Application Notes | WB:1:100~500 IF |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
