Application
XRCC5 Antibody (Center K439) western blot analysis in Jurkat cell line lysates (35ug/lane).This demonstrates the XRCC5 antibody detected the XRCC5 protein (arrow).Application
Confocal immunofluorescent analysis of XRCC5 Antibody (Center K439)(Cat#AP11960c) with hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red). DAPI was used to stain the cell nuclear (blue).Application
XRCC5 Antibody (Center K439) flow cytometric analysis of Jurkat cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.| Product Name | XRCC5 Antibody (Center K439) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | XRCC5; G22P2; X-ray repair cross-complementing protein 5; 86 kDa subunit of Ku antigen; ATP-dependent DNA helicase 2 subunit 2; ATP-dependent DNA helicase II 80 kDa subunit; CTC box-binding factor 85 kDa subunit; DNA repair protein XRCC5; Ku80; Ku86; Lupus |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIFFC |
| WB:1:100~500 IF |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | G22P2 |
|---|---|
| Alternative Names | XRCC5 G22P2 X-ray repair cross-complementing protein 5 86 kDa subunit of Ku antigen ATP-dependent DNA helicase 2 subunit 2 ATP-dependent DNA helicase II 80 kDa subunit CTC box-binding factor 85 kDa subunit DNA repair protein XRCC5 Ku80 Ku86 Lupus |
| Molecular Weight(MW) | 82705 Da |
| Function | Single stranded DNA-dependent ATP-dependent helicase. Has a role in chromosome translocation. The DNA helicase II complex binds preferentially to fork-like ends of double-stranded DNA in a cell cycle-dependent manner. It works in the 3'-5' direction. Binding to DNA may be mediated by XRCC6. Involved in DNA non-homologous end joining (NHEJ) required for double-strand break repair and V(D)J recombination. The XRCC5/6 dimer acts as regulatory subunit of the DNA-dependent protein kinase complex DNA-PK by increasing the affinity of the catalytic subunit PRKDC to DNA by 100-fold. The XRCC5/6 dimer is probably involved in stabilizing broken DNA ends and bringing them together. The assembly of the DNA-PK complex to DNA ends is required for the NHEJ ligation step. In association with NAA15, the XRCC5/6 dimer binds to the osteocalcin promoter and activates osteocalcin expression. The XRCC5/6 dimer probably also acts as a 5'- deoxyribose-5-phosphate lyase (5'-dRP lyase), by catalyzing the beta-elimination of the 5' deoxyribose-5-phosphate at an abasic site near double-strand breaks. XRCC5 probably acts as the catalytic subunit of 5'-dRP activity, and allows to 'clean' the termini of abasic sites, a class of nucleotide damage commonly associated with strand breaks, before such broken ends can be joined. The XRCC5/6 dimer together with APEX1 acts as a negative regulator of transcription |
| Tissue Specificity | This XRCC5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 431-458 amino acids from the Central region of human XRCC5. |
| Cellular Localization | Nucleus. Nucleus, nucleolus. Chromosome. |
Application
XRCC5 Antibody (Center K439) western blot analysis in Jurkat cell line lysates (35ug/lane).This demonstrates the XRCC5 antibody detected the XRCC5 protein (arrow).
Application
Confocal immunofluorescent analysis of XRCC5 Antibody (Center K439)(Cat#AP11960c) with hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red). DAPI was used to stain the cell nuclear (blue).
Application
XRCC5 Antibody (Center K439) flow cytometric analysis of Jurkat cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:100~500 IF |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
