Application
A. Western blot analysis on (A)A549 treated with FBS, (B) HeLa, (C) U937 and (D) HepG2 cell lysates using anti-Ku80 RabMAb (Cat. #AJ1431a), dilution 1:2000.Application
B. Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-Ku80 RabMAb (Cat. #AJ1431a).| Product Name | Ku80 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | XRCC5, G22P2, ATP-dependent DNA helicase 2 subunit 2, Nuclear factor IV;DNA repair protein XRCC5 |
| Clonality | Monoclonal |
|---|---|
| Clone Number | EPR3467 |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:10000 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | G22P2 |
|---|---|
| Alternative Names | XRCC5 G22P2 ATP-dependent DNA helicase 2 subunit 2 Nuclear factor IV DNA repair protein XRCC5 |
| Molecular Weight(MW) | 82705 Da |
| Function | Single stranded DNA-dependent ATP-dependent helicase. Has a role in chromosome translocation. The DNA helicase II complex binds preferentially to fork-like ends of double-stranded DNA in a cell cycle-dependent manner. It works in the 3'-5' direction. Binding to DNA may be mediated by XRCC6. Involved in DNA non-homologous end joining (NHEJ) required for double-strand break repair and V(D)J recombination. The XRCC5/6 dimer acts as regulatory subunit of the DNA-dependent protein kinase complex DNA-PK by increasing the affinity of the catalytic subunit PRKDC to DNA by 100-fold. The XRCC5/6 dimer is probably involved in stabilizing broken DNA ends and bringing them together. The assembly of the DNA-PK complex to DNA ends is required for the NHEJ ligation step. In association with NAA15, the XRCC5/6 dimer binds to the osteocalcin promoter and activates osteocalcin expression. The XRCC5/6 dimer probably also acts as a 5'- deoxyribose-5-phosphate lyase (5'-dRP lyase), by catalyzing the beta-elimination of the 5' deoxyribose-5-phosphate at an abasic site near double-strand breaks. XRCC5 probably acts as the catalytic subunit of 5'-dRP activity, and allows to 'clean' the termini of abasic sites, a class of nucleotide damage commonly associated with strand breaks, before such broken ends can be joined. The XRCC5/6 dimer together with APEX1 acts as a negative regulator of transcription |
| Tissue Specificity | A synthetic peptide corresponding to residues in human Ku80 was used as an immunogen. |
| Cellular Localization | Nucleus. Nucleus, nucleolus. Chromosome. |
| Entrez Gene | 7520 |
|---|
Application
A. Western blot analysis on (A)A549 treated with FBS, (B) HeLa, (C) U937 and (D) HepG2 cell lysates using anti-Ku80 RabMAb (Cat. #AJ1431a), dilution 1:2000.
Application
B. Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-Ku80 RabMAb (Cat. #AJ1431a).| Application Notes | WB:1:10000 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. |
