Application
Figure 1: Western blot analysis using anti-YES1 monoclonal antibody against truncated YES1-His recombinant protein(1) and full-length GFP-YES1(aa1-543) transfected COS7 cell lysate(2).| Product Name | YES1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Yes; c-yes; HsT441; P61-YES; YES1 |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 2F3E6 |
| Host Species | Mouse |
| Tested Applications | WB |
| WB:1:500 - 2000. |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | YES |
|---|---|
| Alternative Names | Yes c-yes HsT441 P61-YES YES1 |
| Molecular Weight(MW) | 60801 Da |
| Function | Non-receptor protein tyrosine kinase that is involved in the regulation of cell growth and survival, apoptosis, cell-cell adhesion, cytoskeleton remodeling, and differentiation Stimulation by receptor tyrosine kinases (RTKs) including EGRF, PDGFR, CSF1R and FGFR leads to recruitment of YES1 to the phosphorylated receptor, and activation and phosphorylation of downstream substrates. Upon EGFR activation, promotes the phosphorylation of PARD3 to favor epithelial tight junction assembly. Participates in the phosphorylation of specific junctional components such as CTNND1 by stimulating the FYN and FER tyrosine kinases at cell-cell contacts. Upon T-cell stimulation by CXCL12, phosphorylates collapsin response mediator protein 2/DPYSL2 and induces T-cell migration. Participates in CD95L/FASLG signaling pathway and mediates AKT-mediated cell migration. Plays a role in cell cycle progression by phosphorylating the cyclin-dependent kinase 4/CDK4 thus regulating the G1 phase. Also involved in G2/M progression and cytokinesis |
| Tissue Specificity | Expressed in the epithelial cells of renal proximal tubules and stomach as well as hematopoietic cells in the bone marrow and spleen in the fetal tissues. In adult, expressed in epithelial cells of the renal proximal tubules and present in keratinocytes in the basal epidermal layer of epidermis |
| Cellular Localization | Cell membrane. Cytoplasm, cytoskeleton, centrosome. Cytoplasm, cytosol. Note=Newly synthesized protein initially accumulates in the Golgi region and traffics to the plasma membrane through the exocytic pathway |
| Entrez Gene | 7525 |
|---|---|
| Nucleotide Accession | NP_005424.1 |
Application
Figure 1: Western blot analysis using anti-YES1 monoclonal antibody against truncated YES1-His recombinant protein(1) and full-length GFP-YES1(aa1-543) transfected COS7 cell lysate(2).| Application Notes | WB:1:500 - 2000. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified recombinant fragment of YES(aa10-193) expressed in E. Coli. |
