Application
ZEB1 Antibody (Ascites) (Cat. #AM1878a) western blot analysis in A549 cell line lysates (35ug/lane).This demonstrates the ZEB1 antibody detected the ZEB1 protein (arrow).Application
ZEB1 Antibody ( Ascites) (Cat. #AM1878a) flow cytometric analysis of A549 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | ZEB1 antibody ( Ascites) |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 213CT14.1.5 |
| Host Species | Mouse |
| Tested Applications | WBFC |
| WB:1:500~16000 FC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | AREB6, TCF8 |
|---|---|
| Alternative Names | ZEB1 AREB6 TCF8 Zinc finger E-box-binding homeobox 1 NIL-2-A zinc finger protein Negative regulator of IL2 Transcription factor 8 |
| Molecular Weight(MW) | 124074 Da |
| Function | Acts as a transcriptional repressor. Inhibits interleukin-2 (IL-2) gene expression. Enhances or represses the promoter activity of the ATP1A1 gene depending on the quantity of cDNA and on the cell type. Represses E-cadherin promoter and induces an epithelial-mesenchymal transition (EMT) by recruiting SMARCA4/BRG1. Represses BCL6 transcription in the presence of the corepressor CTBP1. Positively regulates neuronal differentiation Represses RCOR1 transcription activation during neurogenesis Represses transcription by binding to the E box (5'-CANNTG-3') Promotes tumorigenicity by repressing stemness-inhibiting microRNAs |
| Tissue Specificity | Colocalizes with SMARCA4/BRG1 in E-cadherin- negative cells from established lines, and stroma of normal colon as well as in de-differentiated epithelial cells at the invasion front of colorectal carcinomas (at protein level). Expressed in heart and skeletal muscle, but not in liver, spleen, or pancreas |
| Cellular Localization | Nucleus. |
| Entrez Gene | 6935 |
|---|
Application
ZEB1 Antibody (Ascites) (Cat. #AM1878a) western blot analysis in A549 cell line lysates (35ug/lane).This demonstrates the ZEB1 antibody detected the ZEB1 protein (arrow).
Application
ZEB1 Antibody ( Ascites) (Cat. #AM1878a) flow cytometric analysis of A549 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:500~16000 FC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Mouse monoclonal antibody supplied in crude ascites with 0.09% (W/V) sodium azide. |
