Application
ZIC3 Antibody (Center) western blot analysis in CEM cell line lysates (35ug/lane).This demonstrates the ZIC3 antibody detected the ZIC3 protein (arrow).Application
ZIC3 Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the ZIC3 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.Application
ZIC3 Antibody (Center) flow cytometric analysis of CEM cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | ZIC3 Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCFC |
| WB:1:50~100 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | ZNF203 |
|---|---|
| Alternative Names | ZIC3 ZNF203 Zinc finger protein ZIC 3 Zinc finger protein 203 Zinc finger protein of the cerebellum 3 |
| Molecular Weight(MW) | 50569 Da |
| Function | Acts as transcriptional activator. Required in the earliest stages in both axial midline development and left-right (LR) asymmetry specification. Binds to the minimal GLI-consensus sequence 5'-GGGTGGTC-3' |
| Tissue Specificity | This ZIC3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 246-276 amino acids from the Central region of human ZIC3. |
| Cellular Localization | Nucleus. Cytoplasm (By similarity). Note=Localizes in the cytoplasm in presence of MDFIC overexpression (By similarity). Translocation to the nucleus requires KPNA1 or KPNA6 |
| Entrez Gene | 7547 |
|---|---|
| Nucleotide Accession | NP_003404.1 |
Application
ZIC3 Antibody (Center) western blot analysis in CEM cell line lysates (35ug/lane).This demonstrates the ZIC3 antibody detected the ZIC3 protein (arrow).
Application
ZIC3 Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the ZIC3 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
Application
ZIC3 Antibody (Center) flow cytometric analysis of CEM cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:50~100 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
