Application
Western blot analysis of anti-ZIC3 Antibody (N-term) in HL60 cell line lysates (35ug/lane).ZIC3(arrow) was detected using the purified Pab (1:60dilution).Application
Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with ZIC3 antibody (N-term) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.| Product Name | ZIC3 Antibody (N-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:50~100 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | ZNF203 |
|---|---|
| Alternative Names | ZIC3 ZNF203 Zinc finger protein ZIC 3 Zinc finger protein 203 Zinc finger protein of the cerebellum 3 |
| Molecular Weight(MW) | 50569 Da |
| Function | Acts as transcriptional activator. Required in the earliest stages in both axial midline development and left-right (LR) asymmetry specification. Binds to the minimal GLI-consensus sequence 5'-GGGTGGTC-3' |
| Tissue Specificity | This ZIC3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 11~40 amino acids from the N-terminal region of human ZIC3. |
| Cellular Localization | Nucleus. Cytoplasm (By similarity). Note=Localizes in the cytoplasm in presence of MDFIC overexpression (By similarity). Translocation to the nucleus requires KPNA1 or KPNA6 |
| Entrez Gene | 7547 |
|---|
Application
Western blot analysis of anti-ZIC3 Antibody (N-term) in HL60 cell line lysates (35ug/lane).ZIC3(arrow) was detected using the purified Pab (1:60dilution).
Application
Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with ZIC3 antibody (N-term) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.| Application Notes | WB:1:50~100 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
