Application
Western blot analysis of ZW10 over-expressed 293 cell line, cotransfected with ZW10 Validated Chimera RNAi ( Cat # H00009183-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with ZW10 monoclonal antibody (M05), clone 2E5 (Cat # AT4649a ). GAPDH ( 36.1 kDa ) used as specificity and loading control.Application
Western Blot analysis of ZW10 expression in transfected 293T cell line by ZW10 monoclonal antibody (M05), clone 2E5.Lane 1: ZW10 transfected lysate(88.8 KDa).Lane 2: Non-transfected lysate.| Product Name | ZW10 Antibody (monoclonal) (M05) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | HZW10, KNTC1APZW10, kinetochore associated, homolog (Drosophila) |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2a |
| Clone Number | 200000 |
| Host Species | Mouse |
| Tested Applications | WB |
| WB:1:500~1000 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | HZW10 KNTC1APZW10 kinetochore associated homolog (Drosophila) |
|---|---|
| Molecular Weight(MW) | 88829 Da |
| Tissue Specificity | ZW10 (NP_004715, 1 a.a. ~ 100 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. |
Application
Western blot analysis of ZW10 over-expressed 293 cell line, cotransfected with ZW10 Validated Chimera RNAi ( Cat # H00009183-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with ZW10 monoclonal antibody (M05), clone 2E5 (Cat # AT4649a ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
Application
Western Blot analysis of ZW10 expression in transfected 293T cell line by ZW10 monoclonal antibody (M05), clone 2E5.Lane 1: ZW10 transfected lysate(88.8 KDa).Lane 2: Non-transfected lysate.| Application Notes | WB:1:500~1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Clear, colorless solution in phosphate buffered saline, pH 7.2 . |
