Application
Figure 1: Western blot analysis using anti-4E-BP1 monoclonal antiobdy against truncated 4E-BP1 recombinant protein(1) and A431 cell lysate(2)Application
Figure 2: Immunohistochemical analysis of paraffin-embedded human pancreas carcinoma(A), esophagus carcinoma tissue(B) and ovary tumor tissue(C), showing cytoplasmic and membrane localization using anti-4E-BP1 antibody with DAB staining.| Product Name | 4E-BP1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | BP-1; 4EBP1; 4E-BP1; PHAS-I; MGC4316; EIF4EBP1 |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 4B6G10 |
| Host Species | Mouse |
| Tested Applications | WBIHC |
| WB:1:500 - 2000. IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | BP-1 4EBP1 4E-BP1 PHAS-I MGC4316 EIF4EBP1 |
|---|---|
| Molecular Weight(MW) | 12580 Da |
| Function | Regulates eIF4E activity by preventing its assembly into the eIF4F complex. Mediates the regulation of protein translation by hormones, growth factors and other stimuli that signal through the MAP kinase and mTORC1 pathways |
| Tissue Specificity | Purified recombinant fragment of 4E-BP1 expressed in E. Coli. |
| Entrez Gene | 1978 |
|---|---|
| Nucleotide Accession | NP_004086.1 |
Application
Figure 1: Western blot analysis using anti-4E-BP1 monoclonal antiobdy against truncated 4E-BP1 recombinant protein(1) and A431 cell lysate(2)
Application
Figure 2: Immunohistochemical analysis of paraffin-embedded human pancreas carcinoma(A), esophagus carcinoma tissue(B) and ovary tumor tissue(C), showing cytoplasmic and membrane localization using anti-4E-BP1 antibody with DAB staining.| Application Notes | WB:1:500 - 2000. IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified recombinant fragment of 4E-BP1 expressed in E. Coli. |
