Application
Western blot analysis of ABCG2 Antibody (C-term) in HepG2 cell line lysates (35ug/lane). ABCG2(arrow) was detected using the purified Pab (1:60 dilution).Application
ABCG2 (BCRP) Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human placenta tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of ABCG2 (BCRP) Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.Application
Flow cytometric analysis of HepG2 cells using ABCG2 (BCRP) Antibody (C-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | ABCG2 (BCRP) Antibody (C-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | ABCG2; MXR; ABCP; BCRP; BCRP1; ATP-binding cassette sub-family G member 2; Breast cancer resistance protein; CDw338; Mitoxantrone resistance-associated protein; Placenta-specific ATP-binding cassette transporter; CD_antigen=CD338 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCFC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | ABCP BCRP BCRP1 MXR |
|---|---|
| Alternative Names | ABCG2 MXR ABCP BCRP BCRP1 ATP-binding cassette sub-family G member 2 Breast cancer resistance protein CDw338 Mitoxantrone resistance-associated protein Placenta-specific ATP-binding cassette transporter CD_antigen=CD338 |
| Molecular Weight(MW) | 72314 Da |
| Function | Xenobiotic transporter that may play an important role in the exclusion of xenobiotics from the brain. May be involved in brain-to-blood efflux. Appears to play a major role in the multidrug resistance phenotype of several cancer cell lines. When overexpressed, the transfected cells become resistant to mitoxantrone, daunorubicin and doxorubicin, display diminished intracellular accumulation of daunorubicin, and manifest an ATP- dependent increase in the efflux of rhodamine 123 |
| Tissue Specificity | Highly expressed in placenta. Low expression in small intestine, liver and colon |
| Cellular Localization | Cell membrane; Multi-pass membrane protein. |
| Entrez Gene | 9429 |
|---|
Application
Western blot analysis of ABCG2 Antibody (C-term) in HepG2 cell line lysates (35ug/lane). ABCG2(arrow) was detected using the purified Pab (1:60 dilution).
Application
ABCG2 (BCRP) Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human placenta tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of ABCG2 (BCRP) Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
Application
Flow cytometric analysis of HepG2 cells using ABCG2 (BCRP) Antibody (C-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
