Application
Western blot analysis of ABCG2 Antibody (Center) in 293 cell line lysates (35ug/lane). ABCG2(arrow) was detected using the purified Pab (1:60 dilution).Application
Confocal immunofluorescent analysis of ABCG2 (BCRP) Antibody (Center)(Cat#AP1490c) with HepG2 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).Application
ABCG2 (BCRP) Antibody (Center) flow cytometric analysis of HepG2 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | ABCG2 (BCRP) Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | ABCG2; MXR; ABCP; BCRP; BCRP1; ATP-binding cassette sub-family G member 2; Breast cancer resistance protein; CDw338; Mitoxantrone resistance-associated protein; Placenta-specific ATP-binding cassette transporter; CD_antigen=CD338 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIFFC |
| WB:1:100~500 IF |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | ABCP BCRP BCRP1 MXR |
|---|---|
| Alternative Names | ABCG2 MXR ABCP BCRP BCRP1 ATP-binding cassette sub-family G member 2 Breast cancer resistance protein CDw338 Mitoxantrone resistance-associated protein Placenta-specific ATP-binding cassette transporter CD_antigen=CD338 |
| Molecular Weight(MW) | 72314 Da |
| Function | Xenobiotic transporter that may play an important role in the exclusion of xenobiotics from the brain. May be involved in brain-to-blood efflux. Appears to play a major role in the multidrug resistance phenotype of several cancer cell lines. When overexpressed, the transfected cells become resistant to mitoxantrone, daunorubicin and doxorubicin, display diminished intracellular accumulation of daunorubicin, and manifest an ATP- dependent increase in the efflux of rhodamine 123 |
| Tissue Specificity | Highly expressed in placenta. Low expression in small intestine, liver and colon |
| Cellular Localization | Cell membrane; Multi-pass membrane protein. |
| Entrez Gene | 9429 |
|---|
Application
Western blot analysis of ABCG2 Antibody (Center) in 293 cell line lysates (35ug/lane). ABCG2(arrow) was detected using the purified Pab (1:60 dilution).
Application
Confocal immunofluorescent analysis of ABCG2 (BCRP) Antibody (Center)(Cat#AP1490c) with HepG2 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).
Application
ABCG2 (BCRP) Antibody (Center) flow cytometric analysis of HepG2 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:100~500 IF |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
