Application
Western blot analysis of ACCN1 Antibody (Center) in NCI-H460 cell line lysates (35ug/lane). ACCN1 (arrow) was detected using the purified Pab.Application
Formalin-fixed and paraffin-embedded human brain tissue reacted with ACCN1 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.Application
ACCN1 Antibody (Center) flow cytometric analysis of WiDr cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | ACCN1 Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | ASIC2; ACCN; ACCN1; BNAC1; MDEG; Acid-sensing ion channel 2; Amiloride-sensitive brain sodium channel; Amiloride-sensitive cation channel 1, neuronal; Amiloride-sensitive cation channel neuronal 1; Brain sodium channel 1; Mammalian degenerin homolog |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCFC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | ACCN ACCN1 BNAC1 MDEG |
|---|---|
| Alternative Names | ASIC2 ACCN ACCN1 BNAC1 MDEG Acid-sensing ion channel 2 Amiloride-sensitive brain sodium channel Amiloride-sensitive cation channel 1 neuronal Amiloride-sensitive cation channel neuronal 1 Brain sodium channel 1 Mammalian degenerin homolog |
| Molecular Weight(MW) | 57709 Da |
| Function | Cation channel with high affinity for sodium, which is gated by extracellular protons and inhibited by the diuretic amiloride. Also permeable for Li(+) and K(+). Generates a biphasic current with a fast inactivating and a slow sustained phase Heteromeric channel assembly seems to modulate |
| Tissue Specificity | Brain and spinal cord. Isoform 1 is also detected in testis, liver, colon and ovary |
| Cellular Localization | Cell membrane; Multi-pass membrane protein (By similarity). Note=Localized at the plasma membrane of neurons, in the soma and punctated peripheral processes (By similarity) |
| Entrez Gene | 40 |
|---|
Application
Western blot analysis of ACCN1 Antibody (Center) in NCI-H460 cell line lysates (35ug/lane). ACCN1 (arrow) was detected using the purified Pab.
Application
Formalin-fixed and paraffin-embedded human brain tissue reacted with ACCN1 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Application
ACCN1 Antibody (Center) flow cytometric analysis of WiDr cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
