Application
Western blot analysis of ACOT8 Antibody (C-term) in mouse liver tissue lysates (35ug/lane). ACOT8 (arrow) was detected using the purified Pab.Application
Formalin-fixed and paraffin-embedded human Prostate carcinoma reacted with ACOT8 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.Application
ACOT8 Antibody (C-term) flow cytometric analysis of MDA-MB435 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | ACOT8 Antibody (C-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | ACOT8; ACTEIII; PTE1; PTE2; Acyl-coenzyme A thioesterase 8; Acyl-CoA thioesterase 8; Choloyl-coenzyme A thioesterase; HIV-Nef-associated acyl-CoA thioesterase; PTE-2; Peroxisomal acyl-coenzyme A thioester hydrolase 1; Peroxisomal long-chain acyl-CoA thioes |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCFC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | ACTEIII PTE1 PTE2 |
|---|---|
| Alternative Names | ACOT8 ACTEIII PTE1 PTE2 Acyl-coenzyme A thioesterase 8 Acyl-CoA thioesterase 8 Choloyl-coenzyme A thioesterase HIV-Nef-associated acyl-CoA thioesterase PTE-2 Peroxisomal acyl-coenzyme A thioester hydrolase 1 Peroxisomal long-chain acyl-CoA thioes |
| Molecular Weight(MW) | 35914 Da |
| Function | Acyl-CoA thioesterases are a group of enzymes that catalyze the hydrolysis of acyl-CoAs to the free fatty acid and coenzyme A (CoASH), providing the potential to regulate intracellular levels of acyl-CoAs, free fatty acids and CoASH. May mediate Nef-induced down-regulation of CD4. Major thioesterase in peroxisomes. Competes with BAAT (Bile acid CoA: amino acid N- acyltransferase) for bile acid-CoA substrate (such as chenodeoxycholoyl-CoA). Shows a preference for medium-length fatty acyl-CoAs (By similarity). May be involved in the metabolic regulation of peroxisome proliferation |
| Tissue Specificity | Detected in a T-cell line (at protein level). Ubiquitous |
| Cellular Localization | Peroxisome. |
| Entrez Gene | 10005 |
|---|
Application
Western blot analysis of ACOT8 Antibody (C-term) in mouse liver tissue lysates (35ug/lane). ACOT8 (arrow) was detected using the purified Pab.
Application
Formalin-fixed and paraffin-embedded human Prostate carcinoma reacted with ACOT8 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Application
ACOT8 Antibody (C-term) flow cytometric analysis of MDA-MB435 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
