Application
ACTA1/?-actin Antibody (C-term) western blot analysis in A549 cell line lysates (35ug/lane).This demonstrates the ACTA1/?-actin antibody detected the ACTA1/?-actin protein (arrow).Application
ACTA1/?-actin Antibody (C-term) (AP14779b)immunohistochemistry analysis in formalin fixed and paraffin embedded human skeletal muscle followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of ACTA1/?-actin Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.Application
Confocal immunofluorescent analysis of ACTA1/?-actin Antibody (C-term)(Cat#AP14779b) with A549 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).Application
ACTA1/?-actin Antibody (C-term) flow cytometric analysis of CEM cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | ACTA1/?-actin Antibody (C-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | ACTA1; ACTA; Actin, alpha skeletal muscle; Alpha-actin-1 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCIFFC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | ACTA |
|---|---|
| Alternative Names | ACTA1 ACTA Actin alpha skeletal muscle Alpha-actin-1 |
| Molecular Weight(MW) | 42051 Da |
| Function | Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells |
| Tissue Specificity | This ACTA1-actin antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 345-376 amino acids from the C-terminal region of human ACTA1/?-actin. |
| Cellular Localization | Cytoplasm, cytoskeleton. |
| Entrez Gene | 58 |
|---|---|
| Nucleotide Accession | NP_001091.1 |
Application
ACTA1/?-actin Antibody (C-term) western blot analysis in A549 cell line lysates (35ug/lane).This demonstrates the ACTA1/?-actin antibody detected the ACTA1/?-actin protein (arrow).
Application
ACTA1/?-actin Antibody (C-term) (AP14779b)immunohistochemistry analysis in formalin fixed and paraffin embedded human skeletal muscle followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of ACTA1/?-actin Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
Application
Confocal immunofluorescent analysis of ACTA1/?-actin Antibody (C-term)(Cat#AP14779b) with A549 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).
Application
ACTA1/?-actin Antibody (C-term) flow cytometric analysis of CEM cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
