Application
ACTA1 Antibody(Cat. #AM1965b) western blot analysis in CEM cell line lysates (35ug/lane).This demonstrates the ACTA1 antibody detected the ACTA1 protein (arrow).Application
ACTA1 Antibody (Cat. #AM1965b)immunohistochemistry analysis in formalin fixed and paraffin embedded human colon carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of ACTA1 Antibody for immunohistochemistry. Clinical relevance has not been evaluated.Application
ACTA1 Antibody (Cat. #AM1965b)immunohistochemistry analysis in formalin fixed and paraffin embedded human skeletal muscle followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of ACTA1 Antibody for immunohistochemistry. Clinical relevance has not been evaluated.Application
ACTA1 Antibody (Cat. #AM1965b) flow cytometric analysis of A549 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | ACTA1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | ACTA1; ACTA; Actin, alpha skeletal muscle; Alpha-actin-1 |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 337CT30.10.1 |
| Host Species | Mouse |
| Tested Applications | WBIHCFC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | ACTA |
|---|---|
| Alternative Names | ACTA1 ACTA Actin alpha skeletal muscle Alpha-actin-1 |
| Molecular Weight(MW) | 42051 Da |
| Function | Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells |
| Tissue Specificity | This ACTA1 monoclonal antibody is generated from mouse immunized with ACTA1 recombinant protein. |
| Cellular Localization | Cytoplasm, cytoskeleton. |
| Entrez Gene | 58 |
|---|---|
| Nucleotide Accession | NP_001091.1 |
Application
ACTA1 Antibody(Cat. #AM1965b) western blot analysis in CEM cell line lysates (35ug/lane).This demonstrates the ACTA1 antibody detected the ACTA1 protein (arrow).
Application
ACTA1 Antibody (Cat. #AM1965b)immunohistochemistry analysis in formalin fixed and paraffin embedded human colon carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of ACTA1 Antibody for immunohistochemistry. Clinical relevance has not been evaluated.
Application
ACTA1 Antibody (Cat. #AM1965b)immunohistochemistry analysis in formalin fixed and paraffin embedded human skeletal muscle followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of ACTA1 Antibody for immunohistochemistry. Clinical relevance has not been evaluated.
Application
ACTA1 Antibody (Cat. #AM1965b) flow cytometric analysis of A549 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. |
