Application
Western blot analysis of ACTB/ACTC Antibody (N-term) in mouse testis tissue lysates (35ug/lane). Target protein (arrow) was detected using the purified Pab (1:60 dilution).Application
Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with ACTB/ACTC antibody (N-term) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.Application
Confocal immunofluorescent analysis of Actin (ACTB/ACTC) Antibody (N-term)(Cat#AP1491a) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).| Product Name | Actin (ACTB/ACTC) Antibody (N-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | ACTC1; ACTC; Actin, alpha cardiac muscle 1; Alpha-cardiac actin |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCIF |
| WB:1:50~100 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | ACTC |
|---|---|
| Alternative Names | ACTC1 ACTC Actin alpha cardiac muscle 1 Alpha-cardiac actin |
| Molecular Weight(MW) | 42019 Da |
| Function | Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells |
| Tissue Specificity | This ACTB/ACTC antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 43~73 amino acids from the N-terminal region of human ACTB/ACTC. |
| Cellular Localization | Cytoplasm, cytoskeleton. |
| Entrez Gene | 70 |
|---|
Application
Western blot analysis of ACTB/ACTC Antibody (N-term) in mouse testis tissue lysates (35ug/lane). Target protein (arrow) was detected using the purified Pab (1:60 dilution).
Application
Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with ACTB/ACTC antibody (N-term) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Application
Confocal immunofluorescent analysis of Actin (ACTB/ACTC) Antibody (N-term)(Cat#AP1491a) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).| Application Notes | WB:1:50~100 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
