Application
A. Western blot analysis on A431 cell lysate using anti-Actin RabMAb (Cat. #AJ1012a), dilution 1:1,000Application
B. Immunohistochemical staining of paraffin-embedded human cardiac muscle using anti-Actin RabMAb (Cat. #AJ1012a).Application
C. Immunohistochemical analysis of paraffin-embedded human skeletal muscle using anti-Actin (Pan) RabMAb (Cat. #AJ1012a).Application
D. Immunofluorescent staining of HeLa cells using anti-Actin RabMAb (Cat. #AJ1012a).| Product Name | Actin Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | ACTC1, ACTC, Actin, alpha cardiac muscle 1, Alpha-cardiac actin |
| Clonality | Monoclonal |
|---|---|
| Clone Number | EP184E |
| Host Species | Rabbit |
| Tested Applications | WBIHCIF |
| WB:1:10000~20000 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | ACTC |
|---|---|
| Alternative Names | ACTC1 ACTC Actin alpha cardiac muscle 1 Alpha-cardiac actin |
| Molecular Weight(MW) | 42019 Da |
| Function | Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells |
| Tissue Specificity | A synthetic peptide corresponding to residues near the N-terminus of human Actin was used as an immunogen. This Actin antibody recognizes all muscle actins. |
| Cellular Localization | Cytoplasm, cytoskeleton. |
| Entrez Gene | 70 |
|---|
Application
A. Western blot analysis on A431 cell lysate using anti-Actin RabMAb (Cat. #AJ1012a), dilution 1:1,000
Application
B. Immunohistochemical staining of paraffin-embedded human cardiac muscle using anti-Actin RabMAb (Cat. #AJ1012a).
Application
C. Immunohistochemical analysis of paraffin-embedded human skeletal muscle using anti-Actin (Pan) RabMAb (Cat. #AJ1012a).
Application
D. Immunofluorescent staining of HeLa cells using anti-Actin RabMAb (Cat. #AJ1012a).| Application Notes | WB:1:10000~20000 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. |
