Application
A. Western blot analysis on A549 cell lysates using anti- Phospho-Actopaxin (pS8) RabMAb (Cat. #AJ1013a), 1:5000 dilution. Cells were either (A) untreated (B) treated with LPApplication
B. Immunofluorescent staining of HeLa cells using Phospho-specific anti-Actopaxin RabMAb (Cat. #AJ1013a).| Product Name | Actopaxin/Parvin Antibody Phospho (pS8) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Parva, Actp, Alpha-parvin, Actopaxin |
| Modification | p-S8 |
| Clonality | Monoclonal |
|---|---|
| Clone Number | EPR2174 |
| Host Species | Rabbit |
| Tested Applications | WBIF |
| WB:1:5000~10000 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | Actp |
|---|---|
| Alternative Names | Parva Actp Alpha-parvin Actopaxin |
| Molecular Weight(MW) | 42330 Da |
| Function | Plays a role in the reorganization of the actin cytoskeleton, formation of lamellipodia and ciliogenesis. Plays a role in the establishement of cell polarity, cell adhesion, cell spreading, and directed cell migration. Plays a role in sarcomere organization and in smooth muscle cell contraction. Required for normal development of the embryonic cardiovascular system, and for normal septation of the heart outflow tract. Plays a role in sprouting angiogenesis and is required for normal adhesion of vascular smooth muscle cells to endothelial cells during blood vessel development |
| Tissue Specificity | A phospho specific peptide corresponding to residues surrounding serine 8 of human Parvin was used as an immunogen. This antibody detects Actopaxin phosphorylated on serine 8. |
| Cellular Localization | Cell junction, focal adhesion. Cell membrane; Peripheral membrane protein; Cytoplasmic side Cytoplasm, cytoskeleton. Cytoplasm, myofibril, sarcomere, Z line Note=Constituent of focal adhesions |
| Entrez Gene | 57342 |
|---|
Application
A. Western blot analysis on A549 cell lysates using anti- Phospho-Actopaxin (pS8) RabMAb (Cat. #AJ1013a), 1:5000 dilution. Cells were either (A) untreated (B) treated with LP
Application
B. Immunofluorescent staining of HeLa cells using Phospho-specific anti-Actopaxin RabMAb (Cat. #AJ1013a).| Application Notes | WB:1:5000~10000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. |
