Application
Western blot analysis of extracts from 293 cells using AFX Antibody (S197) (#AE1004a, Lane 1 and 2) and Phospho-AFX-S197 Antibody (#AE1004b, Lane 3 and 4).Application
Immunohistochemical analysis of paraffin-embeddedhuman lung carcinoma tissue, using AFX Antibody (S197) (#AE1004a).| Product Name | AFX Antibody (S197) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Fork head domain transcription factor AFX1; Forkhead box protein O4 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:500~1:1000 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | AFX AFX1 MLLT7 |
|---|---|
| Alternative Names | Fork head domain transcription factor AFX1 Forkhead box protein O4 |
| Molecular Weight(MW) | 53684 Da |
| Function | Transcription factor involved in the regulation of the insulin signaling pathway. Binds to insulin-response elements (IREs) and can activate transcription of IGFBP1. Down-regulates expression of HIF1A and suppresses hypoxia-induced transcriptional activation of HIF1A-modulated genes. Also involved in negative regulation of the cell cycle. Involved in increased proteasome activity in embryonic stem cells (ESCs) by activating expression of PSMD11 in ESCs, leading to enhanced assembly of the 26S proteasome, followed by higher proteasome activity |
| Tissue Specificity | Heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas. Isoform zeta is most abundant in the liver, kidney, and pancreas |
| Cellular Localization | Cytoplasm. Nucleus. Note=When phosphorylated, translocated from nucleus to cytoplasm Dephosphorylation triggers nuclear translocation Monoubiquitination increases nuclear localization. When deubiquitinated, translocated from nucleus to cytoplasm |
| Entrez Gene | 4303 |
|---|
Application
Western blot analysis of extracts from 293 cells using AFX Antibody (S197) (#AE1004a, Lane 1 and 2) and Phospho-AFX-S197 Antibody (#AE1004b, Lane 3 and 4).
Application
Immunohistochemical analysis of paraffin-embeddedhuman lung carcinoma tissue, using AFX Antibody (S197) (#AE1004a).| Application Notes | WB:1:500~1:1000 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | affinity Purified IgG, in PBS, 0.02% sodium azide and 50% glycerol. |
