Application
Western blot analysis of hAGL-C371 in K562 cell line lysates (35ug/lane). AGL (arrow) was detected using the purified Pab.Application
Confocal immunofluorescent analysis of AGL Antibody (Center)(Cat#AP2402a) with HepG2 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).| Product Name | AGL Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIF |
| WB:1:50~100 IF |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | GDE |
|---|---|
| Alternative Names | AGL GDE Glycogen debranching enzyme Glycogen debrancher 4-alpha-glucanotransferase Oligo-1 4-1 4-glucantransferase Amylo-alpha-1 6-glucosidase Dextrin 6-alpha-D-glucosidase |
| Molecular Weight(MW) | 174764 Da |
| Function | Multifunctional enzyme acting as 1,4-alpha-D-glucan:1,4- alpha-D-glucan 4-alpha-D-glycosyltransferase and amylo-1,6- glucosidase in glycogen degradation |
| Tissue Specificity | Liver, kidney and lymphoblastoid cells express predominantly isoform 1; whereas muscle and heart express not only isoform 1, but also muscle-specific isoform mRNAs (isoforms 2, 3 and 4). Isoforms 5 and 6 are present in both liver and muscle |
| Cellular Localization | Cytoplasm. Note=Under glycogenolytic conditions localizes to the nucleus |
| Entrez Gene | 178 |
|---|
Application
Western blot analysis of hAGL-C371 in K562 cell line lysates (35ug/lane). AGL (arrow) was detected using the purified Pab.
Application
Confocal immunofluorescent analysis of AGL Antibody (Center)(Cat#AP2402a) with HepG2 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).| Application Notes | WB:1:50~100 IF |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. |
