Application
A. Western blot analysis of K562 cell lysate using anti-AIF RabMAb (Cat. #AJ1021a), 1:1,000 dilution.Application
B. Immunohistochemical analysis of paraffin-embedded human cervical carcinoma using anti-AIF RabMAb (Cat. #AJ1021a).Application
C. Immunofluorescent staining of HeLa cells, using AIF RabMAb. (Green fluorescence- AIF localized in mitochondria, Red - PI nuclear staining). (Cat. #AJ1021a).Application
D. Flow cytometric analysis of permeabilized K562 cells using anti-AIF RabMAb (red) (Cat. #AJ1021a) or a rabbit IgG (negative)(green).| Product Name | AIF Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | Monoclonal |
|---|---|
| Clone Number | E20 |
| Host Species | Rabbit |
| Tested Applications | WBIHCIFFC |
| WB:1:2500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | AIF, PDCD8 |
|---|---|
| Alternative Names | AIFM1 AIF PDCD8 Apoptosis-inducing factor 1 mitochondrial Programmed cell death protein 8 |
| Molecular Weight(MW) | 66901 Da |
| Function | Probable oxidoreductase that has a dual role in controlling cellular life and death; during apoptosis, it is translocated from the mitochondria to the nucleus to function as a proapoptotic factor in a caspase-independent pathway, while in normal mitochondria, it functions as an antiapoptotic factor via its oxidoreductase activity. The soluble form (AIFsol) found in the nucleus induces 'parthanatos' i.e. caspase-independent fragmentation of chromosomal DNA. Interacts with EIF3G,and thereby inhibits the EIF3 machinery and protein synthesis, and activates casapse-7 to amplify apoptosis. Plays a critical role in caspase- independent, pyknotic cell death in hydrogen peroxide-exposed cells. Binds to DNA in a sequence-independent manner |
| Tissue Specificity | Isoform 5 is frequently down-regulated in human cancers |
| Cellular Localization | Mitochondrion intermembrane space. Mitochondrion inner membrane. Cytoplasm. Nucleus. Cytoplasm, perinuclear region. Note=Proteolytic cleavage during or just after translocation into the mitochondrial intermembrane space (IMS) results in the formation of an inner-membrane-anchored mature form (AIFmit). During apoptosis, further proteolytic processing leads to a mature form, which is confined to the mitochondrial IMS in a soluble form (AIFsol). AIFsol is released to the cytoplasm in response to specific death signals, and translocated to the nucleus, where it induces nuclear apoptosis. Colocalizes with EIF3G in the nucleus and perinuclear region |
| Entrez Gene | 9131 |
|---|
Application
A. Western blot analysis of K562 cell lysate using anti-AIF RabMAb (Cat. #AJ1021a), 1:1,000 dilution.
Application
B. Immunohistochemical analysis of paraffin-embedded human cervical carcinoma using anti-AIF RabMAb (Cat. #AJ1021a).
Application
C. Immunofluorescent staining of HeLa cells, using AIF RabMAb. (Green fluorescence- AIF localized in mitochondria, Red - PI nuclear staining). (Cat. #AJ1021a).
Application
D. Flow cytometric analysis of permeabilized K562 cells using anti-AIF RabMAb (red) (Cat. #AJ1021a) or a rabbit IgG (negative)(green).| Application Notes | WB:1:2500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. |
