Application
Fluorescent image of U251 cells stained with AIFM1 (N-term) antibody. U251 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with AP8910a AIFM1 (N-term) primary antibody (1:200, 2 h at room temperature). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (5.25 μM, 25 min). Pictures were taken on a Biorevo microscope (BZ-900, Keyence). Nuclei were counterstained with Hoechst 33342 (blue) (10 μg/ml, 5 min). AIFM1 (N-term) immunoreactivity is localized to the cytoplasm of U251 cells.Application
Fluorescent confocal image of U251 cells stained with AIFM1 (N-term) antibody. U251 cells were treated with Chloroquine (50 μM,16h), then fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with AP8910a AIFM1 (N-term) primary antibody (1:200, 2 h at room temperature). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 μg/ml, 5 min). AIFM1 (N-term) immunoreactivity is localized to the cytoplasm of U251 cells.Application
Western blot analysis of AIFM1 Antibody (N-term) in 293 cell line lysates (35ug/lane). AIFM1 (arrow) was detected using the purified Pab.Application
Formalin-fixed and paraffin-embedded human brain tissue reacted with AIFM1 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.Application
AIFM1 Antibody (N-term) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | AIFM1 Antibody (N-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IFWBIHCFC |
| IF:1:200 WB |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | AIF, PDCD8 |
|---|---|
| Alternative Names | AIFM1 AIF PDCD8 Apoptosis-inducing factor 1 mitochondrial Programmed cell death protein 8 |
| Molecular Weight(MW) | 66901 Da |
| Function | Probable oxidoreductase that has a dual role in controlling cellular life and death; during apoptosis, it is translocated from the mitochondria to the nucleus to function as a proapoptotic factor in a caspase-independent pathway, while in normal mitochondria, it functions as an antiapoptotic factor via its oxidoreductase activity. The soluble form (AIFsol) found in the nucleus induces 'parthanatos' i.e. caspase-independent fragmentation of chromosomal DNA. Interacts with EIF3G,and thereby inhibits the EIF3 machinery and protein synthesis, and activates casapse-7 to amplify apoptosis. Plays a critical role in caspase- independent, pyknotic cell death in hydrogen peroxide-exposed cells. Binds to DNA in a sequence-independent manner |
| Tissue Specificity | Isoform 5 is frequently down-regulated in human cancers |
| Cellular Localization | Mitochondrion intermembrane space. Mitochondrion inner membrane. Cytoplasm. Nucleus. Cytoplasm, perinuclear region. Note=Proteolytic cleavage during or just after translocation into the mitochondrial intermembrane space (IMS) results in the formation of an inner-membrane-anchored mature form (AIFmit). During apoptosis, further proteolytic processing leads to a mature form, which is confined to the mitochondrial IMS in a soluble form (AIFsol). AIFsol is released to the cytoplasm in response to specific death signals, and translocated to the nucleus, where it induces nuclear apoptosis. Colocalizes with EIF3G in the nucleus and perinuclear region |
| Entrez Gene | 9131 |
|---|
Application
Fluorescent image of U251 cells stained with AIFM1 (N-term) antibody. U251 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with AP8910a AIFM1 (N-term) primary antibody (1:200, 2 h at room temperature). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (5.25 μM, 25 min). Pictures were taken on a Biorevo microscope (BZ-900, Keyence). Nuclei were counterstained with Hoechst 33342 (blue) (10 μg/ml, 5 min). AIFM1 (N-term) immunoreactivity is localized to the cytoplasm of U251 cells.
Application
Fluorescent confocal image of U251 cells stained with AIFM1 (N-term) antibody. U251 cells were treated with Chloroquine (50 μM,16h), then fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with AP8910a AIFM1 (N-term) primary antibody (1:200, 2 h at room temperature). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 μg/ml, 5 min). AIFM1 (N-term) immunoreactivity is localized to the cytoplasm of U251 cells.
Application
Western blot analysis of AIFM1 Antibody (N-term) in 293 cell line lysates (35ug/lane). AIFM1 (arrow) was detected using the purified Pab.
Application
Formalin-fixed and paraffin-embedded human brain tissue reacted with AIFM1 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Application
AIFM1 Antibody (N-term) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | IF:1:200 WB |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
