Application
AIF1 Antibody (C-term) western blot analysis in CEM cell line lysates (35ug/lane).This demonstrates the AIF1 antibody detected the AIF1 protein (arrow).Application
AIF1 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded mouse heart tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of AIF1 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.Application
AIF1 Antibody (C-term) flow cytometric analysis of CEM cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | AIF1 Antibody (C-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCFC |
| WB:1:50~100 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | G1, IBA1 |
|---|---|
| Alternative Names | AIF1 G1 IBA1 Allograft inflammatory factor 1 Ionized calcium-binding adapter molecule 1 Protein G1 |
| Molecular Weight(MW) | 16703 Da |
| Function | Actin-binding protein that enhances membrane ruffling and RAC activation. Enhances the actin-bundling activity of LCP1 Binds calcium. Plays a role in RAC signaling and in phagocytosis May play a role in macrophage activation and function. Promotes the proliferation of vascular smooth muscle cells and of T- lymphocytes. Enhances lymphocyte migration. Plays a role in vascular inflammation |
| Tissue Specificity | Detected in T-lymphocytes and peripheral blood mononuclear cells |
| Cellular Localization | Cytoplasm, cytoskeleton. Cell projection, ruffle membrane; Peripheral membrane protein; Cytoplasmic side Note=Associated with the actin cytoskeleton at membrane ruffles and at sites of phagocytosis |
| Entrez Gene | 199 |
|---|---|
| Nucleotide Accession | NP_001614.3 |
Application
AIF1 Antibody (C-term) western blot analysis in CEM cell line lysates (35ug/lane).This demonstrates the AIF1 antibody detected the AIF1 protein (arrow).
Application
AIF1 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded mouse heart tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of AIF1 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
Application
AIF1 Antibody (C-term) flow cytometric analysis of CEM cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:50~100 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
