Application
Western blot analysis of AKAP10 over-expressed 293 cell line, cotransfected with AKAP10 Validated Chimera RNAi ( Cat # H00011216-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with AKAP10 monoclonal antibody (M04), clone 8C10 (Cat # AT1082a ). GAPDH ( 36.1 kDa ) used as specificity and loading control.Application
Western Blot analysis of AKAP10 expression in transfected 293T cell line by AKAP10 monoclonal antibody (M04), clone 8C10.Lane 1: AKAP10 transfected lysate(73.8 KDa).Lane 2: Non-transfected lysate.| Product Name | AKAP10 Antibody (monoclonal) (M04) |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2a |
| Clone Number | 8C10 |
| Host Species | Mouse |
| Tested Applications | WB |
| WB:1:500~1000 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | DAKAP2 MGC9414 PRKA10A kinase (PRKA) anchor protein 10 |
|---|---|
| Molecular Weight(MW) | 73818 Da |
| Tissue Specificity | AKAP10 (NP_009133, 1 a.a. ~ 101 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. |
Application
Western blot analysis of AKAP10 over-expressed 293 cell line, cotransfected with AKAP10 Validated Chimera RNAi ( Cat # H00011216-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with AKAP10 monoclonal antibody (M04), clone 8C10 (Cat # AT1082a ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
Application
Western Blot analysis of AKAP10 expression in transfected 293T cell line by AKAP10 monoclonal antibody (M04), clone 8C10.Lane 1: AKAP10 transfected lysate(73.8 KDa).Lane 2: Non-transfected lysate.| Application Notes | WB:1:500~1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Clear, colorless solution in phosphate buffered saline, pH 7.2 . |
