Application
AMFR Antibody (Center) western blot analysis in T47D cell line lysates (35ug/lane).This demonstrates the AMFR antibody detected the AMFR protein (arrow).| Product Name | AMFR Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | AMFR; RNF45; E3 ubiquitin-protein ligase AMFR; Autocrine motility factor receptor, isoform 2; RING finger protein 45; gp78 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:100~500 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | RNF45 |
|---|---|
| Alternative Names | AMFR RNF45 E3 ubiquitin-protein ligase AMFR Autocrine motility factor receptor isoform 2 RING finger protein 45 gp78 |
| Molecular Weight(MW) | 72996 Da |
| Function | E3 ubiquitin-protein ligase that mediates the polyubiquitination of a number of proteins such as CD3D, CYP3A4, CFTR and APOB for proteasomal degradation. Component of a VCP/p97- AMFR/gp78 complex that participates in the final step of endoplasmic reticulum-associated degradation (ERAD). The VCP/p97- AMFR/gp78 complex is involved in the sterol-accelerated ERAD degradation of HMGCR through binding to the HMGCR-INSIG complex at the ER membrane and initiating ubiquitination of HMGCR. The ubiquitinated HMGCR is then released from the ER by the complex into the cytosol for subsequent destruction. Also acts as a scaffold protein to assemble a complex that couples ubiquitination, retranslocation and deglycosylation. Mediates tumor invasion and metastasis as a receptor for the GPI/autocrine motility factor |
| Tissue Specificity | This AMFR antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 370-400 amino acids from the Central region of human AMFR. |
| Cellular Localization | Endoplasmic reticulum membrane; Multi-pass membrane protein |
| Entrez Gene | 267 |
|---|---|
| Nucleotide Accession | NP_001135.3 |
Application
AMFR Antibody (Center) western blot analysis in T47D cell line lysates (35ug/lane).This demonstrates the AMFR antibody detected the AMFR protein (arrow).| Application Notes | WB:1:100~500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
