Application
Western blot analysis of Annexin A1 antibody (C-term) in NCI-H460 cell line lysates (35ug/lane). Annexin A1 (arrow) was detected using the purified Pab.Application
Annexin A1 Antibody (C-term) IHC analysis in formalin fixed and paraffin embedded human lung carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the Annexin A1 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.Application
Confocal immunofluorescent analysis of Annexin A1 Antibody (C-term)(Cat#AP6584b) with A2058 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).DAPI was used to stain the cell nuclear (blue).| Product Name | Annexin A1 Antibody (C-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | ANXA1; ANX1; LPC1; Annexin A1; Annexin I; Annexin-1; Calpactin II; Calpactin-2; Chromobindin-9; Lipocortin I; Phospholipase A2 inhibitory protein; p35 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCIF |
| WB:1:50~100 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | ANX1 LPC1 |
|---|---|
| Alternative Names | ANXA1 ANX1 LPC1 Annexin A1 Annexin I Annexin-1 Calpactin II Calpactin-2 Chromobindin-9 Lipocortin I Phospholipase A2 inhibitory protein p35 |
| Molecular Weight(MW) | 38714 Da |
| Function | Calcium/phospholipid-binding protein which promotes membrane fusion and is involved in exocytosis. This protein regulates phospholipase A2 activity. It seems to bind from two to four calcium ions with high affinity |
| Tissue Specificity | This Annexin A1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 226~255 amino acids from the C-terminal region of human Annexin A1. |
| Cellular Localization | Nucleus (By similarity). Cytoplasm (By similarity). Cell projection, cilium (By similarity). Basolateral cell membrane (By similarity). Note=Found in the cilium, nucleus and basolateral cell membrane of ciliated cells in the tracheal endothelium (By similarity). Found in the cytoplasm of type II pneumocytes and alveolar macrophages (By similarity) |
| Entrez Gene | 301 |
|---|
Application
Western blot analysis of Annexin A1 antibody (C-term) in NCI-H460 cell line lysates (35ug/lane). Annexin A1 (arrow) was detected using the purified Pab.
Application
Annexin A1 Antibody (C-term) IHC analysis in formalin fixed and paraffin embedded human lung carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the Annexin A1 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
Application
Confocal immunofluorescent analysis of Annexin A1 Antibody (C-term)(Cat#AP6584b) with A2058 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).DAPI was used to stain the cell nuclear (blue).| Application Notes | WB:1:50~100 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
