Application
Immunofluorescence of monoclonal antibody to APC on HeLa cell . [antibody concentration 10 ug/ml]Application
Proximity Ligation Analysis (PLA) of protein-protein interactions between JUP and APC. HeLa cells were stained with anti-JUP rabbit purified polyclonal 1:1200 and anti-APC mouse monoclonal antibody 1:50. Signals were detected by Duolink® 30 Detection Kit 613(red), and nuclei were counterstained with DAPI (blue). Each red dot represents the detection of protein-protein interaction complex.Application
Western Blot detection against Immunogen (37.22 KDa) .| Product Name | APC Antibody (monoclonal) (M01) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | DP2, DP2.5, DP3, FAP, FPC, GSadenomatosis polyposis coli |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 300 |
| Host Species | Mouse |
| Tested Applications | IFWB |
| WB:1:500~1000 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | DP2 DP2.5 DP3 FAP FPC GSadenomatosis polyposis coli |
|---|---|
| Molecular Weight(MW) | 311646 Da |
| Tissue Specificity | APC (NP_000029, 2744 a.a. ~ 2845 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. |
Application
Immunofluorescence of monoclonal antibody to APC on HeLa cell . [antibody concentration 10 ug/ml]
Application
Proximity Ligation Analysis (PLA) of protein-protein interactions between JUP and APC. HeLa cells were stained with anti-JUP rabbit purified polyclonal 1:1200 and anti-APC mouse monoclonal antibody 1:50. Signals were detected by Duolink® 30 Detection Kit 613(red), and nuclei were counterstained with DAPI (blue). Each red dot represents the detection of protein-protein interaction complex.
Application
Western Blot detection against Immunogen (37.22 KDa) .| Application Notes | WB:1:500~1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Clear, colorless solution in phosphate buffered saline, pH 7.2 . |
