Application
hAPG7L-D555 (PEI 1:100)b western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the APG7L antibody detected the APG7L protein (arrow).Application
APG7L Antibody(D555) immunohistochemistry analysis in formalin fixed and paraffin embedded human liver tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of APG7L Antibody(D555) for immunohistochemistry. Clinical relevance has not been evaluated.Application
APG7L Antibody flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | APG7L Antibody(D555) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | ATG7; APG7L; Ubiquitin-like modifier-activating enzyme ATG7; ATG12-activating enzyme E1 ATG7; Autophagy-related protein 7; Ubiquitin-activating enzyme E1-like protein |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCFC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | APG7L |
|---|---|
| Alternative Names | ATG7 APG7L Ubiquitin-like modifier-activating enzyme ATG7 ATG12-activating enzyme E1 ATG7 Autophagy-related protein 7 Ubiquitin-activating enzyme E1-like protein |
| Molecular Weight(MW) | 77960 Da |
| Function | Functions as an E1 enzyme essential for multisubstrates such as ATG8-like proteins and ATG12. Forms intermediate conjugates with ATG8-like proteins (GABARAP, GABARAPL1, GABARAPL2 or MAP1LC3A). PE-conjugation to ATG8-like proteins is essential for autophagy. Also acts as an E1 enzyme for ATG12 conjugation to ATG5 and ATG3 (By similarity) |
| Tissue Specificity | Widely expressed, especially in kidney, liver, lymph nodes and bone marrow |
| Cellular Localization | Cytoplasm (Probable). |
| Entrez Gene | 10533 |
|---|---|
| Nucleotide Accession | NP_006386.1 |
Application
hAPG7L-D555 (PEI 1:100)b western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the APG7L antibody detected the APG7L protein (arrow).
Application
APG7L Antibody(D555) immunohistochemistry analysis in formalin fixed and paraffin embedded human liver tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of APG7L Antibody(D555) for immunohistochemistry. Clinical relevance has not been evaluated.
Application
APG7L Antibody flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
