Application
A. Western blot analysis on Jurkat cell lysate using anti-Atg7 RabMAb (Cat. #AJ1065a).Application
B. Immunohistochemical analysis of paraffin-embedded human liver using anti-Atg7 RabMAb (Cat. #AJ1065a).| Product Name | Atg7 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | Monoclonal |
|---|---|
| Clone Number | EP1759Y |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:10000~50000 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | APG7L |
|---|---|
| Alternative Names | ATG7 APG7L Autophagy-related protein 7 APG7-like Ubiquitin-activating enzyme E1-like protein |
| Molecular Weight(MW) | 77960 Da |
| Function | Functions as an E1 enzyme essential for multisubstrates such as ATG8-like proteins and ATG12. Forms intermediate conjugates with ATG8-like proteins (GABARAP, GABARAPL1, GABARAPL2 or MAP1LC3A). PE-conjugation to ATG8-like proteins is essential for autophagy. Also acts as an E1 enzyme for ATG12 conjugation to ATG5 and ATG3 (By similarity) |
| Tissue Specificity | Widely expressed, especially in kidney, liver, lymph nodes and bone marrow |
| Cellular Localization | Cytoplasm (Probable). |
| Entrez Gene | 10533 |
|---|
Application
A. Western blot analysis on Jurkat cell lysate using anti-Atg7 RabMAb (Cat. #AJ1065a).
Application
B. Immunohistochemical analysis of paraffin-embedded human liver using anti-Atg7 RabMAb (Cat. #AJ1065a).| Application Notes | WB:1:10000~50000 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. |
