Application
Western blot analysis of AQR Antibody (Center) in CEM cell line lysates (35ug/lane). AQR (arrow) was detected using the purified Pab;Application
Western blot analysis of AQR Antibody (Center) in mouse brain tissue lysates (35ug/lane). AQR (arrow) was detected using the purified Pab.Application
Formalin-fixed and paraffin-embedded human brain tissue reacted with AQR Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.Application
AQR Antibody (Center) flow cytometry analysis of CEM cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | AQR Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | AQR; KIAA0560; Intron-binding protein aquarius; Intron-binding protein of 160 kDa |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCFC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | KIAA0560 |
|---|---|
| Alternative Names | AQR KIAA0560 Intron-binding protein aquarius Intron-binding protein of 160 kDa |
| Molecular Weight(MW) | 171295 Da |
| Function | Intron-binding spliceosomal protein required to link pre-mRNA splicing and snoRNP (small nucleolar ribonucleoprotein) biogenesis. Plays a key role in position-dependent assembly of intron-encoded box C/D small snoRNP, splicing being required for snoRNP assembly. May act by helping the folding of the snoRNA sequence. Binds to intron of pre-mRNAs in a sequence-independent manner, contacting the region between snoRNA and the branchpoint of introns (40 nucleotides upstream of the branchpoint) during the late stages of splicing |
| Tissue Specificity | This AQR antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 539~568 amino acids from the Center region of human AQR. |
| Cellular Localization | Nucleus. Note=Localizes to speckle-like regions of the nucleoplasm |
| Entrez Gene | 9716 |
|---|
Application
Western blot analysis of AQR Antibody (Center) in CEM cell line lysates (35ug/lane). AQR (arrow) was detected using the purified Pab;
Application
Western blot analysis of AQR Antibody (Center) in mouse brain tissue lysates (35ug/lane). AQR (arrow) was detected using the purified Pab.
Application
Formalin-fixed and paraffin-embedded human brain tissue reacted with AQR Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Application
AQR Antibody (Center) flow cytometry analysis of CEM cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
