Application
Fluorescent image of U251 cells stained with AM1817a ATG16L1 antibody.U251 cells were treated with Chloroquine (50 μM,16h), then fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with AM1817a ATG16L1 primary antibody (1:200, 2 h at room temperature). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-mouse antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 μg/ml, 5 min). ATG16L1 immunoreactivity is localized to autophagic vacuoles in the cytoplasm of U251 cells, supported by Human Protein Atlas Data (http://www.proteinatlas.org/ENSG00000085978).Application
Western blot analysis of anti-ATG16L1 Monoclonal Antibody (Cat.#AM1817a) by ATG16L1 recombinant protein (Fragment). ATG16L1 (Fragment) protein (arrow) was detected using the ascites Mab. (1:2000)| Product Name | ATG16L1 Antibody (Ascites) |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 54CT27.2.6 |
| Host Species | Mouse |
| Tested Applications | IFWB |
| IF:1:200 WB |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | APG16L |
|---|---|
| Alternative Names | ATG16L1 APG16L Autophagy-related protein 16-1 APG16-like 1 |
| Molecular Weight(MW) | 68265 Da |
| Function | Plays an essential role in autophagy: interacts with ATG12-ATG5 to mediate the conjugation of phosphatidylethanolamine (PE) to LC3 (MAP1LC3A, MAP1LC3B or MAP1LC3C), to produce a membrane-bound activated form of LC3 named LC3-II |
| Tissue Specificity | Purified His-tagged ATG16L1 protein(Fragment) was used to produced this monoclonal antibody. |
| Cellular Localization | Cytoplasm (By similarity). Preautophagosomal structure membrane; Peripheral membrane protein (By similarity) Note=Localized to preautophagosomal structure (PAS) where it is involved in the membrane targeting of ATG5 (By similarity) |
| Entrez Gene | 55054 |
|---|
Application
Fluorescent image of U251 cells stained with AM1817a ATG16L1 antibody.U251 cells were treated with Chloroquine (50 μM,16h), then fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with AM1817a ATG16L1 primary antibody (1:200, 2 h at room temperature). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-mouse antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 μg/ml, 5 min). ATG16L1 immunoreactivity is localized to autophagic vacuoles in the cytoplasm of U251 cells, supported by Human Protein Atlas Data (http://www.proteinatlas.org/ENSG00000085978).
Application
Western blot analysis of anti-ATG16L1 Monoclonal Antibody (Cat.#AM1817a) by ATG16L1 recombinant protein (Fragment). ATG16L1 (Fragment) protein (arrow) was detected using the ascites Mab. (1:2000)| Application Notes | IF:1:200 WB |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Mouse monoclonal antibody supplied in crude ascites with 0.09% (W/V) sodium azide. |
