Application
ATG4D/MB10250 antibody (Cat. #AM1896b) western blot analysis in HL-60 cell line lysates (35ug/lane).This demonstrates the ATG4D/MB10250 antibody detected the ATG4D/MB10250 protein (arrow).Application
Confocal immunofluorescent analysis of ATG4D Antibody (Cat#AM1896b) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-mouse lgG (green). DAPI was used to stain the cell nuclear (blue).| Product Name | ATG4D Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 222CT15.4.1 |
| Host Species | Mouse |
| Tested Applications | WBIF |
| WB:1:100~1000 IF |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | APG4D, AUTL4 |
|---|---|
| Alternative Names | ATG4D APG4D AUTL4 Cysteine protease ATG4D AUT-like 4 cysteine endopeptidase Autophagin-4 Autophagy-related cysteine endopeptidase 4 Autophagy-related protein 4 homolog D |
| Molecular Weight(MW) | 52922 Da |
| Function | Cysteine protease required for autophagy, which cleaves the C-terminal part of either MAP1LC3, GABARAPL2 or GABARAP, allowing the liberation of form I. A subpopulation of form I is subsequently converted to a smaller form (form II). Form II, with a revealed C-terminal glycine, is considered to be the phosphatidylethanolamine (PE)-conjugated form, and has the capacity for the binding to autophagosomes |
| Tissue Specificity | Mainly expressed in skeletal muscle and, to a lower extent, in testis |
| Cellular Localization | Cytoplasm (Probable). |
| Entrez Gene | 84971 |
|---|---|
| Nucleotide Accession | NP_116274.3 |
Application
ATG4D/MB10250 antibody (Cat. #AM1896b) western blot analysis in HL-60 cell line lysates (35ug/lane).This demonstrates the ATG4D/MB10250 antibody detected the ATG4D/MB10250 protein (arrow).
Application
Confocal immunofluorescent analysis of ATG4D Antibody (Cat#AM1896b) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-mouse lgG (green). DAPI was used to stain the cell nuclear (blue).| Application Notes | WB:1:100~1000 IF |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. |
