Application
Western blot analysis of anti-ATG7 Antibody (Center) Pab in 293 cell line lysates transiently transfected with the ATG7 gene (2ug/lane). hAPG7L-P299(arrow) was detected using the purified Pab (1:30 dilution).Application
The ATG7 Antibody (Center) Pab is used in Western blot to detect APG7L in mouse liver tissue lysate. APG7L (arrow) was detected using the purified Pab.Application
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.| Product Name | ATG7 Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | APG7L |
|---|---|
| Alternative Names | ATG7 APG7L Ubiquitin-like modifier-activating enzyme ATG7 ATG12-activating enzyme E1 ATG7 Autophagy-related protein 7 Ubiquitin-activating enzyme E1-like protein |
| Molecular Weight(MW) | 77960 Da |
| Function | Functions as an E1 enzyme essential for multisubstrates such as ATG8-like proteins and ATG12. Forms intermediate conjugates with ATG8-like proteins (GABARAP, GABARAPL1, GABARAPL2 or MAP1LC3A). PE-conjugation to ATG8-like proteins is essential for autophagy. Also acts as an E1 enzyme for ATG12 conjugation to ATG5 and ATG3 (By similarity) |
| Tissue Specificity | Widely expressed, especially in kidney, liver, lymph nodes and bone marrow |
| Cellular Localization | Cytoplasm (Probable). |
| Entrez Gene | 10533 |
|---|
Application
Western blot analysis of anti-ATG7 Antibody (Center) Pab in 293 cell line lysates transiently transfected with the ATG7 gene (2ug/lane). hAPG7L-P299(arrow) was detected using the purified Pab (1:30 dilution).
Application
The ATG7 Antibody (Center) Pab is used in Western blot to detect APG7L in mouse liver tissue lysate. APG7L (arrow) was detected using the purified Pab.
Application
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
