B3GAT1 Antibody (Cat. #AM2042a) western blot analysis in 293 cell line lysates (35ug/lane).This demonstrates the B3GAT1 antibody detected the B3GAT1 protein (arrow).
Involved in the biosynthesis of L2/HNK-1 carbohydrate epitope on glycoproteins. Can also play a role in glycosaminoglycan biosynthesis. Substrates include asialo- orosomucoid (ASOR), asialo-fetuin, and asialo-neural cell adhesion molecule. Requires sphingomyelin for activity: stearoyl- sphingomyelin was the most effective, followed by palmitoyl- sphingomyelin and lignoceroyl-sphingomyelin. Activity was demonstrated only for sphingomyelin with a saturated fatty acid and not for that with an unsaturated fatty acid, regardless of the length of the acyl group (By similarity)
Tissue Specificity
Mainly expressed in the brain.
Cellular Localization
Golgi apparatus membrane; Single-pass type II membrane protein
B3GAT1 Antibody (Cat. #AM2042a) western blot analysis in 293 cell line lysates (35ug/lane).This demonstrates the B3GAT1 antibody detected the B3GAT1 protein (arrow).
Application Notes
WB:1:100~250
Additional Information
Form
Liquid
Storage Instructions
For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Storage Buffer
Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Euglobin precipitation followed by dialysis against PBS.