Application
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.Application
Bak Antibody (BH3) western blot analysis in Jurkat,MDA-MB453,293 cell line lysates (35ug/lane).This demonstrates the Bak antibody detected the Bak protein (arrow).| Product Name | Bak Antibody (BH3 Domain Specific) |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IHCWB |
| IHC:1:50~100 WB |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | BAK, BCL2L7, CDN1 |
|---|---|
| Alternative Names | BAK1 BAK BCL2L7 CDN1 Bcl-2 homologous antagonist/killer Apoptosis regulator BAK Bcl-2-like protein 7 |
| Molecular Weight(MW) | 23409 Da |
| Function | In the presence of an appropriate stimulus, accelerates programmed cell death by binding to, and antagonizing the anti- apoptotic action of BCL2 or its adenovirus homolog E1B 19k protein. Low micromolar levels of zinc ions inhibit the promotion of apoptosis |
| Tissue Specificity | Expressed in a wide variety of tissues, with highest levels in the heart and skeletal muscle |
| Cellular Localization | Mitochondrion membrane; Single-pass membrane protein (Potential) |
| Entrez Gene | 578 |
|---|
Application
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
Application
Bak Antibody (BH3) western blot analysis in Jurkat,MDA-MB453,293 cell line lysates (35ug/lane).This demonstrates the Bak antibody detected the Bak protein (arrow).| Application Notes | IHC:1:50~100 WB |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. |
