Application
Western blot analysis of BCL2L1(arrow) using rabbit polyclonal BCL2L1 antibody (N-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the BCL2L1 gene (Lane 2) (Origene Technologies).Application
Formalin-fixed and paraffin-embedded human brain tissue reacted with BCL2L1 antibody (N-term) (Cat.# AP7958a), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.Application
Confocal immunofluorescent analysis of BCL2L1 Antibody (N-term)(Cat#AP7958a) with NCI-H460 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).DAPI was used to stain the cell nuclear (blue).| Product Name | BCL2L1 Antibody (N-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | BCL2L1; BCL2L; BCLX; Bcl-2-like protein 1; Apoptosis regulator Bcl-X |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCIF |
| WB:1:50~100 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | BCL2L BCLX |
|---|---|
| Alternative Names | BCL2L1 BCL2L BCLX Bcl-2-like protein 1 Apoptosis regulator Bcl-X |
| Molecular Weight(MW) | 26049 Da |
| Function | Potent inhibitor of cell death. Inhibits activation of caspases (By similarity). Appears to regulate cell death by blocking the voltage-dependent anion channel (VDAC) by binding to it and preventing the release of the caspase activator, CYC1, from the mitochondrial membrane. Also acts as a regulator of G2 checkpoint and progression to cytokinesis during mitosis |
| Tissue Specificity | Bcl-X(S) is expressed at high levels in cells that undergo a high rate of turnover, such as developing lymphocytes. In contrast, Bcl-X(L) is found in tissues containing long-lived postmitotic cells, such as adult brain |
| Cellular Localization | Mitochondrion membrane; Single-pass membrane protein. Nucleus membrane; Single-pass membrane protein; Cytoplasmic side (By similarity). Cytoplasm, cytoskeleton, centrosome. Note=Mitochondrial membranes and perinuclear envelope (By similarity). Localizes to the centrosome when phosphorylated at Ser-49 |
| Entrez Gene | 598 |
|---|
Application
Western blot analysis of BCL2L1(arrow) using rabbit polyclonal BCL2L1 antibody (N-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the BCL2L1 gene (Lane 2) (Origene Technologies).
Application
Formalin-fixed and paraffin-embedded human brain tissue reacted with BCL2L1 antibody (N-term) (Cat.# AP7958a), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Application
Confocal immunofluorescent analysis of BCL2L1 Antibody (N-term)(Cat#AP7958a) with NCI-H460 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).DAPI was used to stain the cell nuclear (blue).| Application Notes | WB:1:50~100 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
